Objective To detect the expression of S100P, E-cadherin and Ki-67 in human colorectal carcinomas, to analyse their expression correlation and evaluated their clinical significance. Methods The S100P,E-cadherin and Ki-67 expression in the tissue of eighty colorectal carcinoma patients,in their normal intestinal mucosa near the tumor,and in thirty benign in-test disease tissue was detected by immuno-histochemistry and then investigated their correlation with clinical and pathological features and the renlationship among them. Results ①The positive rate of S100P protein in colorectal carcinoma group (57. 5%) was strikingly higher than in the normal tissue (3. 8%) and benign intest disease (6. 7%) groups (X2 = 66. 234, P <0. 01). In lymph node metastasis group and distant organ metastasis group (Dukes C+D), the expression rate of S100P protein was obviously higher than in non-lymph node metastasis group (μ =4. 940, P<0. 05) and early stage group respectively (X2 = 5. 693, P<0. 05). The expression of S100P protein increased in the more poorly differentiation tumor. ②The positive rate of E-cadherin in colorectal carcinoma group (52. 5%) was obviously lower than in the normal tissue (93. 8%) and benign intest disease (90. 0%) groups (X2 =41. 014, P<0. 01). The expression of E-cadherin was significantly correlated with tumor differentiation,lymph node metastasis and clinic staging (P<0. 05). ③The positive index of Ki-67 in colorectal carcinoma group (66. 78% ± 9. 77%) was notably higher than the other two contrast groups (31. 40% ± 9. 19% and 7. 14%±3. 87%)(P<0. 01). In lymph node metastasis group and distant organ metastasis group (Dukes C+D),the positive index of Ki-67 protein became higher than the contrast group,but no obvious difference was observed ( P>0. 05). There was no correlation between the Ki-67 expression and theother clinical and pathological features. ④ Correlation analysis showed that: there was no obvious correlation observed among them (P>0. 05). But can not deny the possibility that they may effect through indirect cooperation. Conclusion S100P and Ki-67 was highly expressed in human colorectal carcinomas, but low level E-cadherin expression was observed. The expression of S100P and E-cadherin was significantly correlated with tumor invasion and metastasis. It is helpful to detect the three index together for predicting tumor progression and prognosis.%目的 研究人结直肠癌组织中S100P,E-cadherin和Ki-67的表达情况并探讨三者表达的关系及临床意义.方法 应用免疫组织化学SP法检测80例结直肠癌及其癌旁正常肠黏膜组织、30例良性肠病中三者的表达情况,结合患者临床病理资料进行分析,并探讨三者间表达的相关性.结果 ①S100P蛋白在结直肠癌组织中的表达率(57.5％)显著高于良性肠病(6.7％)及癌旁正常组织(3.8％)(x2=66.234,P＜0.01)；较之对照组,淋巴结转移组(x2=4.940,P＜0.05)及晚期(Dukes C+D期)结直肠癌组(x2=5.693,P＜0.05)S100P蛋白的表达均显著升高；随着结直肠癌分化程度的降低,S100P蛋白的表达有升高的趋势.②结直肠癌组织中E-cadherin的表达率(52.5％)较良性肠病(90.0％)及癌旁正常组织(93.8％％)明显降低(x2=41.014,P＜0.01)；E-cadherin的表达与结直肠癌的分化程度、有无淋巴结转移、临床分期相关(P＜0.05).③Ki-67在结直肠癌组织中的阳性指数(66.78％±9.77％)明显高于两对照组(31.40％±9.19％和7.14％±3.87％)(P＜0.0l)；淋巴结转移组和晚期(Dukes C+D期)组Ki-67阳性指数(68.49％±9.27％和69.35％±8.78％)升高,但较对照组(65.44％±l0.04％和65.74％±10.03％)差异无统计学显著性意义(P＞0.05)；Ki-67表达与其他临床病理特征无关.④三者间表达无显著相关性(P＞0.05),但不能排除在肿瘤进展过程中三者有间接的协同作用.结论 结直肠癌组织中S100P和Ki-67表达明显升高,而E-cadherin表达明显降低；S100P和E-cadherin与结直肠癌组织侵袭、转移密切相关；联合检测三者有助于判断结直肠癌的发展趋势及评估预后.
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