Objective To improve the serum sample for the detection of Epstein-Barr antibodies by ELISA and investigate the application value of the improved method in physical examination screening. Methods The mixed serum method (improved method) was developed by the methods of the classical EBV-CA-IgA and EBV-EA-D-IgA. According to the epidemiological studies of the positive distribution,every two of the physical examination serums were mixed together with an equal amount and then operated as a single specimen according to the original instructions. Results Compared with the classical method (standard method) ,Kappa values of EBV-CA-IgA and EBV-EA-D-IgA for the improved method were 0. 94 and 0. 99, respectively. These two methods had high consistence in screening of EBV-CA-IgA and EBV-EA-D-IgA. The intra-assay precision and inter-assay precision of the improved method were all satisfied ( CV was between 2. 3% ～6. 98%). The bias for cross-interference between the different blood types,interference of rheumatoid factor and interference from non-specific antigen (HBsAg and HBeAg),were all less than 10%. The positive predicted values of EBV-CA-IgA and EBV-EA-D-IgA were 92. 6% and 98. 0% ,respectively,and the negative predicted values were all 100%. Conclusion The improved method is simple,convenient,practical and useful. It can reduce the cost and detection work burden,is worth popularizing.%目的 ELISA法检测EB病毒抗体血清样本优化,并探讨其在体检初筛中的应用价值.方法 在经典EBV-CA-IgA,EBV-EA-D-IgA检测方法的基础上,根据流行病学调查患者阳性分布特点,将体检血清每两人等量混合稀释为一个标本后,按原操作说明书操作.结果 合并血清方法(改良方法)和经典方法(标准方法)进行平行试验,EBV-CA-IgA,EBV-EA-D-IgA的Kappa值分别为0.94和0.99,表示两方法检测结果一致.改良方法的批内、批间精密度较好(CV在2.3%～6.98%之间),不同患者血型间的交叉干扰和类风湿因子干扰以及非特异性抗原(HbsAg,HbeAg)的干扰,偏差均小于10%.EBV-CA-IgA和EBV-EA-D-IgA的阳性预测值分别为92.6%和98.0%,阴性预测值均为100%.结论 优化方法简便、实用、可减少成本和检测工作负担,是值得推广的方法.
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