儿童支气管哮喘患者血浆中miRNA-125b及miRNA-133b的表达及临床意义

摘要

Objective To explore the diagnostic value of the quantitative detection of plasma miRNA-125b and miRNA-133b in children with asthma.Methods Thirty asthmatic patients were enrolled in this study and collected the blood specimens during acute phase and stable phase respectively (AP group and SP group).Thirty allergic rhinitis children (AR group) and thirty healthy children were recruited to the control group (NC group).The levels of miRNA in different groups were detected by qRT-PCR.The performance of miRNA-125b and miRNA-133b were evaluated by receiver operating characteristic curves (ROC) and the area under the curve (AUC) (95％CI).Results The relative expression of miRNA-125b in AP group and SP group were significantly higher than A R group (t=3.913,3.120,P＜0.01),miRNA-133b.In AP group and AR group the expression of miRNA-133b were significantly higher than control group (t=4.426,4.720,P＜0.01).The detection of miRNA-125b yielded an area under the curve of ROC of 0.7989,(95 ％ CI:0.7111～ 0.8864) in discriminating asthmatic patients from healthy group.And the miRNA-133b was 0.7274 (95％CI:0.586 5～0.863 0) in discriminating asthmatic patients during the acute phase from healthy group.Conclusion The relative expression of miRNA-125b in children with asthma was significantly higher than that in AR group and NC group,miRNA-125b may prove to be a non-invasive biomarker for the auxiliary diagnosis of asthma especially when the relative expression up to 1.998.%目的 探讨miRNA-125b和miRNA-133b在支气管哮喘患儿中表达水平及其辅助诊断价值.方法 纳入2016年1月～11月常州市儿童医院、高邮市中医医院30例哮喘患儿,分别收集其急性发作期和稳定期血液标本(AP组,SP组).同期匹配30例变应性鼻炎患儿(AR组)及30例健康儿童为对照(NC组).应用实时荧光定量PCR检测miRNA在各组中的表达水平并相互比较.应用ROC曲线及AUC(95％CI)评估其作为哮喘诊断指标的诊断效能.结果 miRNA-125b在AP组及SP组中的表达量较AR组升高,差异有统计学意义(t=3.913,3.120,P值均＜0.01).miRNA-133b在AP组及AR组中的表达量较高,与NC组相比差异有统计学意义(t=4.426,4.720,P值均＜0.01).miRNA-125b在哮喘诊断中最佳临界值为1.998,敏感度为64.52％,特异度为92.67％,AUC为0.798 9,95％CI为0.711 1～0.886 4;miRNA-133b在哮喘急性期及变应性鼻炎等气道炎症性疾病发作期中诊断最佳临界值为1.667,敏感度为58.06％,特异度为86.67％,AUC为0.727 4,95％CI为0.586 5～0.863 0.结论 哮喘患儿中miRNA-125b表达量较NC组及AR组升高,miRNA-125b可作为哮喘辅助诊断指标,miRNA-133b可用于气道炎症性疾病发作期的辅助诊断.