miR-221与miR-222在甲状腺乳头状癌组织中的表达及临床意义

摘要

Objective To explore the expressions of miR-221 and miR-222 in papillary thyroid carcinoma (PTC),and relativi-ties with clinical pathological features.Methods Samples from patients of PCT (43 cases),nodular golter(21 cases),and para-carcinoma thyroid tissues(14 cases),78 cases in total (from 06/2015 to 05/2016,Shaanxi Provincial People’s Hospital) were collected.Real time-PCR tests were carried out,then analyzed in relation to clinical pathology features,and statistical a-nalysis was used to evaluate the results.Results The expressions of miR-221 and miR-222 were significantly higher in PTC (11.54±3.37,10.67±2.45)than in nodular golter (3.21±1.12,2.89±1.23)and normal thyroid tissue (2.02±0.76, 1.98±0.34)(t=3.62,3.25;3.27,3.01,all P<0.05),which were significantly related with Regional lymph node metasta-sis,tumor invasion coated,distant metastasis and clinical stage (P<0.05),while not related with the gender,age,or size of the tumor of the patients (P>0.05),and no differences were found in nodular golter and in normal thyroid tissue (t=0.91, 0.79,P>0.05).Conclusion miR-221 and miR-222 could be considered as a specific molecular marker of PTC,may play an important role in the diagnosis and treatment on PTC.%目的：探讨miR-221与miR-222在甲状腺乳头状癌（PTC）组织中的表达及其与临床病理因素之间的关系。方法收集陕西省人民医院耳鼻咽喉头颈外科2015年5月～2016年5月就诊的甲状腺乳头状癌（43例）患者、结节性甲状腺肿（21例）及癌旁正常甲状腺组织（14例）共78例，行real time-PCR检测组织中的miR-221及miR-222，并结合临床病理因素进行分析，对结果进行统计学分析。结果甲状腺乳头状癌组中的 miR-221及 miR-222相对表达水平（11.54±3.37，10.67±2.45）高于结节性甲状腺肿组（3.21±1.12，2.89±1.23）及正常对照组（2.02±0.76，1.98±0.34），差异有统计学意义（t＝3.62，3.25；3.27，3.01，均P＜0.05），且与患者的性别、年龄、肿瘤大小等因素无关（P＞0.05），而与局部淋巴结转移、肿瘤侵犯包膜、远处转移以及临床分期相关（P＜0.05）。结节性甲状腺肿组中的 miR-221及 miR-222与正常对照组比较差异无统计学意义（t＝0.91，0.79，P＞0.05）。结论 miR-221及miR-222可作为甲状腺乳头状癌相对特异的分子标志物，对甲状腺乳头状癌的临床诊断及治疗有重要作用。