不同氧分压间歇性暴露对机体免疫功能的影响

摘要

目的：探讨在不同氧分压环境之间进行间歇性暴露,对机体免疫功能的影响。方法：30只雄性BALB/c小鼠随机分为3组,高原富氧间歇性暴露组（E组）,高原对照组（C-H组）和平原对照组（C-L组）。连续暴露14 d,每日12 h,检测小鼠免疫器官指数、淋巴细胞增殖活性;血清免疫球蛋白（IgG）和C-反映蛋白（CRP）水平。结果：与C-L组比较E组和C-H组的胸腺指数降低（P〈0.05）,各组脾脏指数差异无显著性（P〉0.05）;各组IgG、CRP差异无显著性（P〉0.05）;与C-L组相比E组和C-H组T淋巴细胞增殖能力降低,差异有显著性（P〈0.01）;B淋巴细胞增殖能力各组之间差异无显著性（P〉0.05）。结论：间歇性低氧及相对富氧暴露14 d后小鼠特异性细胞免疫功能降低,体液免疫功能相对稳定,但E组损伤和下降的程度比C-H组明显减轻。%Objective： To explore the effect of intermittent exposure under different oxygen pressure on mice immune function.Methods： 30 BALB/c mice were randomly divided into three groups,plain group（C-L group）,hypoxia group（C-H group） and oxygen-enriched group（E group）, and were exposed consecutively for 14 days,12 hours per day.Test the immune organ index,blood CRP and IgG content and proliferative activity of spleen lymphocyte. Results： Compared with C-L group,thymus index of E group and C-H group were decreased（P〈0.05）,while spleen index between three groups had no significant difference.The concentrations of CRP and IgG between three groups had no significant difference.Compared with C-L group,proliferative activity of T lymphocyte of E group and C-H group were significantly decreased（P〈0.01）.Proliferative activities of B lymphocyte between three groups had no significant difference.Conclusion： After 14 days exposure under different oxygen pressure,the mice＇s specific cellular immunity function was depressed,while humoral immune function was relatively stable.However, the damage degree of E group was minor than C-H group.