不同海水浓度和培养时间对海洋真菌抗菌活性的影响

摘要

用滤纸片琼脂扩散法研究不同海水浓度和培养时间对海洋真茵抗菌活性的影响,旨在为大规模培养海洋真菌和提高获得抗菌物质的几率提供理论依据.结果显示:用不同海水浓度培养基发酵海洋真菌,在供试的10株海洋真菌中,5林海洋真菌的抗菌活性和抗菌谱有明显差异；培养时间不同,6株海洋真菌的抗菌活性差别较大.实验结果表明,不同海水浓度和培养时间对海洋真菌抗菌活性有显著影响.%The effects of different seawater concentration and culture time on antimicrobial activity of marine fungi were studied using agar diffusion method with filter paper diskettes, aiming at providing theoretical foundation for cul-turing marine fungi on a large scale and improving the probability of antimicrobial metabolites. The results showed that the differences of antimicrobial activities and antimicrobial spectrum of five among ten tested marine fungal strains cultured in different seawater concentration media were fairly obvious; antimicrobial activities of six marine fungal strains were significantly different at the different culture time. The study indicated different seawater concentration and culture time greatly affected the antimicrobial activity of marine fungi.