人乳头瘤病毒特异性T细胞系细胞冻存后细胞的存活率及功能

摘要

Survival rate and function of human papillomavirus-specific T cell line cells after cryopreservation were in-vestigated applying cryopreserved 6 T cell line cells(5 CD4T cell line,1 CD8 T cell line)containing 10% dimethyl sulfoxide and 90% fetal calf serum cryopreservative liquid. They were recovered after 32 ~ 54 months′cryopreservation in liquid nitrogen,the recovery rate and function of the T cell line cells were investigated using trypan blue staining and ELISPOT(enzyme-linked immunospot assay)respectively. The results showed that the recovery rate of 6 T cell lines varied from 24. 7% to 93. 5% after thawing,and varied form 2. 5% to 72. 2% after overnight incubation. The recovery rate of CD8 T cell line cells was higher than that of CD4 T cell line cells. The 6 T cell line cells were able to secrete IFN-γ after recovery and PHA induction. Therefore,human papillomavirus-specific T cell line cells could sus-tain better survival rate and function after recovery from cryopreservation.%研究人乳头瘤病毒特异性 T 细胞系细胞冻存后细胞的存活率及功能。应用包含10%二甲基亚砜、90%小牛血清的冻存液冻存6个 T 细胞系（5个 CD4 T 细胞系，1个 CD8 T 细胞系）细胞，液氮中冻存32~54个月后复苏，台盼蓝染色法检测复苏后 T 细胞系细胞的存活率，用酶联免疫斑点法（enzyme-linked immunospot assay，ELISPOT）检测复苏后 T 细胞系细胞的功能。结果显示，6个 T 细胞系细胞液氮冻存解冻后细胞的存活率为24.7%~93.5%，过夜培养后细胞的存活率为2.5%~72.2%。CD8 T 细胞系细胞的存活率高于 CD4 T细胞系细胞。6个复苏后的 T 细胞系细胞在 PHA 诱导后均能分泌 IFN-γ。人乳头瘤病毒特异性 T 细胞系细胞冻存复苏后能够保持较好的存活率和功能。