人类免疫缺陷病毒1型同性恋感染者与健康人群Th17/Treg平衡状况的比较研究

摘要

The aim of this study is to characterize the changes in interleukin 17 (IL-17)-secreting T helper cell type 17 (Thl7) cell frequencies and CI4+ CD25hiFoxp3+ regulatory T cell (Treg) in a cohort of chronic human immunodeficiency virus type 1(HIV-1)-infected homosexual patients in China, and to clarify the role of a Th17/Treg imbalance in HIV pathogenesis. A total of 54 untreated chronic HIV-infected individuals and 32 healthy donors were recruited in this study. Peripheral blood mononuclear cells were isolated from EDTA-anticoagulated fresh whole blood and stained to characterize Th17/CD4 and Treg/ CD4. Our results indicated that the depletion of Th17 cells (0.68 ± 0.35 vs 1.42 ± 0.86, P＜0.001) was accompanied by an increase in Treg cells (6.15 ± 2.12 vs 4.50 ± 0.76, P＜0.001), resulting in a loss of ThlT/Treg balance in HIV infection (0.12 ± O. 07 vs O. 31 ± O. 17, P＜O. 001). Additionally, Th17/CD4 was positively correlated to CD4+ T cell counts (r = 0. 371, P＜0.05), and Treg/CD4 was inversely correlated to CD4 + T cell counts and positively correlated to plasma viral load (r= -0.402, P＜O. 05; r =0. 447, P＜0. 001). A positive correlation was observed between Th17/Treg and CD4+ T cell counts, and Th17/Treg was negatively correlated to viral load ( r = 0. 525, P＜0.001; r = - 0. 318, P＜0.05). These results indicate that a loss of immune balance during HIV-1 disease progression may have a critical role in HIV-1 infection and further shed new light into understanding the pathogenesis of HIV-1.%通过探讨人类免疫缺陷病毒1型(HIV-1)同性恋感染者外周血中辅助性T细胞17(Th17)与 CD4+CD25hiFoxp3+调节性T细胞(Treg)比例及Th17/Treg平衡状态与疾病进展的关系,初步阐明Th17/Treg失衡在HIV发病机制中的作用和意义.选取54例未经抗病毒治疗的HIV感染者,另有32名健康志愿者作为正常对照.分离外周血单核细胞后,利用流式细胞技术检测Th17和Treg水平.结果表明,在HIV感染者外周血中Th17比例明显低于正常对照组(0.68±0.35 vs 1.42±0.86,P<0.001),Treg比例明显高于正常对照(6.15±2.12 vs 4.50±0.76,P<0.001),导致HIV感染者中Th17/Treg比例较正常对照显著降低(0.12±0.07 vs 0.31±0.17,P<0.001).研究还发现,Th17/CD4比例与CD4+ T细胞计数正相关(r=0.371,P<0.05),与病毒载量不相关;Treg/CD4比例与CD4 +T细胞计数负相关,与病毒载量正相关(r=-0.402,P<0.05;r=0.447,P<0.001).此外,Th17/Treg比例与CD4+ T细胞计数正相关,与病毒载量负相关(r=0.525,P<0.001;r=-0.318,P<0.05).结果提示,HIV感染中存在Th17/Treg失衡现象,与疾病进程密切相关,可能在HIV进展中具有重要作用.