为研究细菌胞外多聚物（ Extracellular polymeric substances，EPS）的复杂组分及其对环境中矿物细颗粒的溶蚀、改性作用，本文选取了土壤常驻菌硅酸盐细菌、空气常见致病菌金黄色葡萄球菌和人体常见菌大肠杆菌为研究对象，采用反相高效液相色谱（RP - HPLC）快速定性定量检测了三种细菌胞外多糖中单糖成分的方法。结果表明：8种单糖在检测条件下进行了很好的分离，硅酸盐细菌胞外多糖中单糖成分有甘露糖和葡萄糖醛酸；金黄色葡萄球菌胞外多糖中单糖成分有核糖、葡萄糖，且在其生长过程中会消耗甘露糖、葡萄糖醛酸和半乳糖；大肠杆菌胞外多糖中单糖成分有核糖和半乳糖，且在其生长过程中会消耗甘露糖、葡萄糖醛酸、葡萄糖和半乳糖；不同微生物胞外多糖组成和含量上均有较大差异。该研究建立了用反相高效液相色谱快速测定细菌胞外多糖组分的方法。%To identify the complex components of the bacterial extra - cellular polymeric substances(EPS) and their effects of corrosion and surface modified on the mineral fine particles,three common bacteria from soil, air and human body were chosen to be tested strains. They were silicate bacteria,Staphylococcus aureus and Esch-erichia coli. RP - HPLC method was applied to rapid determinate the monosaccharide components of bacterial EPS qualitatively and quantitatively. The results showed 8 monosaccharides were separated well under the detecting condition. The monosaccharides of silicate bacterial EPS included mannose and glucuronic acid,while Staphylo-coccus aureusˊs EPS included ribose and glucose and Escherichia coli EPS included ribose and galactose. The dif-ferent bacteria consumed different monosaccharides in its growth process. The results also showed that the EPS monosaccharide components of each bacteria appeared apparent difference. In this research a method with RP -HPLC was established to determine the monosaccharide components of bacterial EPS.
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