Objective To further verify Bushenhuoxue decoction effects on the repair of rabbit articular cartilage defects using BM-SCs complex . Methods Isolated from a 3 - month - old rabbit by a combination method of density gradient centrifugation and adherent culture,the BMSCs was then implanted into rabbit knee cartilage defects. Chinese medicine Bushenhuoxue decoction was given , which served as Bushenhuoxue decoction + stem cell group. The study also set Bushenhuoxue decoction group, composite group and blank group (control group). Morphological observation and histological examination were performed in repaired tissue at 4,8,12 weeks. Wakitani score was counted to evaluate the repairing effect . Results After 12 weeks,the defects in the Bushenhuoxue decoction + stem cell group was filled with hyaline cartilage - like tissue ;cartilage and subchondral bone tissue were basically repaired; repair of cartilage in histomorphol-ogy was superior to other groups. Conclusion BMSCs complex can repair articular cartilage defects in rabbits, and Chinese medicine Bushenhuoxue decoction can promote cartilage repair and improve the quality of repair.%目的 进一步验证补肾活血汤对骨髓基质干细胞(BMSCs)复合体修复兔关节软骨缺损的影响,评价修复效果.方法 取兔骨髓基质干细胞进行密度离心法结合贴壁培养法体外增殖后,复合于改建后的脱细胞真皮基质(acellular dermal matrix,ADM)载体上,植入到兔膝关节软骨缺损,并以中药补肾活血汤灌胃为复合组,并设立单纯中药组、单纯于细胞复合体组、空白组为对照组.4、8、12周后分别对修复组织进行形态学观察及组织学检查.进行Wakitani评分,观察其体内修复关节缺损效果.结果 术后12周,复合组的缺损由透明软骨样组织充填,软骨及软骨下骨组织基本修复,修复的软骨组织在组织形态学上优于其他各组.结论 骨髓基质干细胞复合体能修复家兔膝关节软骨缺损,中药补肾活血汤能促进软骨的修复,且提高了修复质量.
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