SDF-1/CXCR4的表达与喉癌组织VEGF-C表达、淋巴管生成及预后的相关性

摘要

Objective To investigate the relationship between the expression of SDF - 1 ,CXCR4 and VEGF - C, lymphangiogenesis , prognosis significance in patients with laryngeal cancer. Methods The expressions of SDF - 1, CXCR4 and VEGF-C in 45 specimens of the laryngeal cancer and 20 specimens of polyp of vocal cord tissues were observed by immunohistochemistry. Lymphatic mi-crovessed density( LVD) was evaluated by 5' - Nase staining, and the correlation analysis was made between SDF-1, CXCR4 and VEGF -C, clinical characteristics, and judged SDF - 1 and CXCR4 roles in laryngeal cancer prognosis by using Kaplan - Meier method. Results The expression of SDF - 1 , CXCR4, VEGF - C protein were higher in laryngeal cancer tissue than in the polyp of vocal cord tissue. The expression of SDF-1 and CXCR4 were significantly correlated with TNM stage, lymph node metastasis, lymphovascular invasion. VEGF-C was correlated with lymph node metastasis, lymphovascular invasion. The expression of the SDF-1, CXCR4 and VEGF -C protein in laryngeal cancer has directly correlation. SDF-1 , CXCR4, VEGF-C were significantly correlated with peritumoral and in-tratumoral LVD. The expression of SDF-1 had effect on survival ratio. Conclusion SDF-1/CXCR4 axis might stimulate VEGF - C up-regulation and promote lymphangiogenesis, lymphatic metastasis in laryngeal cell carcinoma. The expression of SDF - 1 may be helpful for judging prognosis in laryngeal cancer.%目的 探讨趋化因子SDF -1及其受体CXCR4在喉癌组织中的表达与喉癌中VEGF-C表达、淋巴管生成及预后的相关性.方法 应用免疫组化法检测SDF -1、CXCR4和VEGF-C在45例喉癌和20例声带息肉组织中的表达,应用5′-核苷酸酶染色法(5′- Nase)计数淋巴管密度,并结合临床病理特征进行相关分析,应用Kaplan -Meier法评估SDF -1和CXCR4表达对喉癌预后的影响.结果 喉癌组织中SDF -1、CXCR4和VEGF -C表达较声带息肉组显著增加(P＜0.05),SDF -1、CXCR4表达与TNM分期、淋巴结转移、淋巴管浸润相关(P＜0.05),VEGF -C表达与淋巴结转移、淋巴管浸润相关(P＜0.05).SDF -1、CXCR4和VEGF -C表达呈正相关(P＜0.05),三者均与瘤内和瘤周淋巴管密度相关(P＜0.05).SDF -1阳性表达生存率显著低于阴性表达(P＜0.05).结论 SDF - 1/CXCR4生物轴可能通过上调VEGF -C表达促进喉癌淋巴管生成和淋巴结转移,SDF -1高表达是喉癌预后不良的重要指标.