脂联素基因多态性与2型糖尿病肾病的关系

摘要

Objective To investigate the association of single nuclcolide polymorphism (SNP) of +45 and + 276 loci of adiponectin ( APN) gene with type 2 diabetic nephropathy (DN) in Han ethnicity population, Zhejiang province in China. Methods Eighty - two type 2 diabetic patients were assigned to normal albiuninuria group, micro - alhummuria group,and macro - albuminuru group according to the urine albumin excretion rate ( UAER). Direct sequencing was used to analyze attele and genotype frequencies of SNP +45 and + 276 loci, and analying the relationship with other observed indicators. Results The distributions of genotype and allele frequencies of SNP +45 and + 276 had no difference among three subgroups (P > 0.05). Conclusion The T→G polymorphism of SNP + 45 and G→T polymorphism of SNP + 276 in adiponectin gene are not associated with type 2 diabetic nephropathy in Han ethnicity population, Zhejiang province in China. The gene polymorphisms of these two loci can not predict the susceptibility to type 2 diabetic nephropathy.%目的 研究脂联素(APN)基因+45位点与+276位点单核苷酸多态性(SNP)与中国浙江地区汉族人群2型糖尿病肾病(DN)的关系.方法 根据尿白蛋白排泄率(UAER)将82例2型糖尿病患者分成糖尿病正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组；用直接测序法分析SNP+ 45和+276两位点基因型和等位基因频率,并分析与其他指标的关系.结果 脂联素基因SNP+45和+276两多态性位点的基因型和等位基因频率分别在正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组3组间无统计学差异(P＞0.05).结论 脂联素基因SNP+45T/G和SNP+276G/T与浙江地区汉族人群中2型糖尿病肾病无相关性.这两位点的基因多态性不能预测2型糖尿病肾病的易感性.