慢性乙型肝炎患者T淋巴细胞及激活亚群与基因型的分析

摘要

Objective To investigate the correlation between genotype and T - lymphocyte and activated T - lymphocyte subsets in chronic hepatitis B. Methods Totally 175 chronic hepatitis B patients and 43 healthy blood donors were enrolled in the study. The T -lymphocyte subsets( CD3 + ,CD3 + CD4 + and CD3 + CD8 + ) and activated T - lymphocyte subsets( CD3 + HLA - DR + 、CD4 + HLA - DR + and CD8 + HLA - DR + ) were detected by flow cytometry. HBV genotypes was detected by a real time fluorescence PCR and HBeAg was detected by chemilumine - scence method. Results Compared with the B genotype HBeAg( + ) group, the percentage of CD8 cells was increased in C genotype patients. While there was no difference in the percentage of CD3 ,CD4 cells and activated T - lymphocyte subsetswith B genotype group and C genotype group. Conclusion There was certain relation between different genotype and cellular immune in chronic hepatitis B patients, especially CD8 cells.%目的 探讨慢性乙型肝炎(CHB)患者不同基因分型(B型和C型)外周血T淋巴细胞及激活亚群的表达情况.方法 收集2008年11月～2010年12月杭州市第六人民医院175例CHB患者,另选取43名健康体检者作为对照.用流式细胞仪检测外周血T淋巴细胞亚群(CD3+、CD3+ CD4+和CD3+ CD8+)及激活亚群(CD3+HLA-DR+、CD4+ HLA - DR+和CD8+ HLA-DR+)的表达,荧光PCR法检测HBV基因型,化学发光法定量检测血清HBeAg含量.结果 B基因型HBeAg(+)组与C基因型组间CD8细胞百分比有显著性差异,C基因型患者CD8细胞高于B基因型.各组间CD3、CD4淋巴细胞及激活亚群均无统计学意义.结论 CHB患者不同基因分型与机体细胞免疫尤其是CD8细胞存在一定的关系.