低剂量饮酒抑制大鼠肝组织DNA中8-oxo-dGsn水平

摘要

Objective To study the level of 8-dihydro-2'-deoxyguanosine (8-oxo-dGsn),a biomarker of DNA oxidation,in the livers of rats after mild alcohol consumption.Methods Totally 48 male Sprague-Dawley (SD) rats at four months of age were allocated randomly in to two groups:the normal control group and the alcohol consumption group.The rats in the alcohol group were given 56° alcohol by intragastric administration in a total dose of 2g/(kg · d) while the the rats in the control group were given the same volume of physiological saline (0.9％,NaCl).At the end of 4,8,12 weeks of treatment,we determined the level of 8-oxo-dGsn,as a biomarker of DNA oxidation,in livers of rats by ID-LC-MS/MS,and meanwhile,detected the ability of agonistic-superoxide anion and the active of glutathion peroxidase (GSH-PX) by spectrophotometric method.Results The level of 8-oxo-dGsn in the liver tissues of alcohol group was lower than that of the control group in 4,8 and 12weeks (P ＜ 0.05).At the same time,the ability of agonistic-superoxide anion(O2-) and the active of glutathion peroxidase (GSH-PX) in the liver tissues of alcohol group were higher than that of control group (P ＜ 0.05).Conclusion These results suggest that a low dosage of alcohol consumption for rats would suppress the DNA oxidation in livers,which may be related to the active of the antioxidative enzyme raised up after drinking slightly.%目的 检测低剂量饮酒大鼠肝组织DNA中氧化标志物8-羟基脱氧鸟苷(8-oxo-dGsn)水平,评价低剂量饮酒对肝脏组织氧化应激的影响.方法 4月龄的健康雄性SD大鼠48只,随机分为对照组和饮酒组,饮酒组每天灌胃56度二锅头酒每天每公斤低于2g(＜2g/kg),对照组每天灌胃同等体积的生理盐水.在灌胃4、8、12周时,用放射性核素稀释高效液相-串联质谱(ID-LC-MS/MS)方法检测大鼠肝组织DNA中氧化标志物8-羟基脱氧鸟苷(8-oxo-dGsn)含量；用分光光度法测定抗超氧阴离子(O2-)能力和谷胱甘肽过氧化物酶(GSH-PX)活力.结果 低剂量饮酒4、8、12周后的肝组织DNA中8-oxo-dGsn含量均较同时间点对照组低,差异有统计学意义(P＜0.05)；低剂量饮酒4、8、12周后的肝组织中抗O2-能力与GSH-PX活性均增强,与对照组相比差异有统计学意义(P＜0.05).结论 低剂量饮酒可抑制大鼠肝组织DNA中的氧化水平,可能与肝脏中抗氧化酶活性的增高有关.