重组KGF-2突变体对TNBS诱导的复发性大鼠炎性肠病模型治疗作用的研究

摘要

Objective To develop a model of recurrent IBD in rats and explore the therapeutic effect of the recombinant KGF-2 mutant (STEA) on the recurrent IBD rat model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS).Methods Male Wister rats were treated with 2％ TNBS (100mg/kg),and were subjected another infusion of TNBS (10mg) three weeks later.Rats in STEA group were administrated IP with 3mg/kg STEA once a day for 7 days after the second infusion of TNBS.At the same time,rats in normal group and control group received equal PB.Body weight,food weight,mortality,the levels of IL-6 and IL-1β in serum and microscopic damage of the colonic tissue were examined.Results The model of recurrent IBD in rats was successfully induced by TNBS.Therapeutic administration of the STEA significantly decreased the levels of IL-6 and IL-1β in serum,reduced the mortality of rats treated with TNBS,and accelerated the wound healing of colonic mucosa.Conclusion Therapeutic administration of the STEA could ameliorate the colonic damages of recurrent IBD rat model induced by TNBS.%目的 制备复发性大鼠炎性肠病模型,评价重组KGF-2突变体(STEA)给药对2,4,6-三硝基苯磺酸(TNBS)诱导的复发性大鼠炎性肠病模型的治疗效果.方法 利用2％的TNBS(100mg/kg)对雄性Wistar大鼠进行灌肠,3周后,再次利用TNBS(10毫克/只)处理这些大鼠.于再次灌肠当天,治疗组的大鼠腹腔注射3mg/kg STEA,模型对照组和正常组注射等量的PB缓冲液,每天1次,连续7天.对各组大鼠的生存状态、血清中IL-6和IL-1β的表达水平、以及结肠组织病理变化等情况进行观察和分析.结果 利用TNBS二次致敏的方法,成功制备了复发性大鼠炎性肠病模型.STEA给药能显著降低模型大鼠血清中IL-6和IL-1β的表达水平,提高大鼠的存活率,促进受损结肠黏膜的修复.结论 STEA对TNBS诱导的复发性大鼠的结肠损伤具有治疗作用.