肉苁蓉炮制前后对大鼠肠神经递质及VIP、SP表达的影响

摘要

Objective To investigate the laxative effect of Cistanche before and after processing.Methods SD rats were filled to give corresponding test solution,to observe the gastrointestinal hormones and neurotransmitters,as well as the change of content and colon tissue of substance P (SP) and vasoactive intestinal peptide (VIP) in the distribution.Results Compared with normal group,the AchE of treatment group significantly increased in serum (P ＜0.01),ET-1 and NO have a tendency to decline.Compared with normal control group,the 5-HT of treatment group significantly increased in colon tissue (P ＜ 0.01,P ＜ 0.05),and AQP3 drops significantly (P ＜0.01,P ＜ 0.05).It can be seen from the colon pathological section diagram,the water high dosage of group and positive medicine group were better than normal group,the treatment group was no significant difference.We can find that colon tissues of normal rats in the VIP,SP immunohistochemical expression of qualitative data,compared with normal control group,the SP of treatment group significantly increased (P ＜ 0.01),VIP had a tendency to rise.Compared with products powder low dose of group,raw water extraction group、raw powder group of SP and VIP increased significantly (P ＜ 0.05,P ＜ 0.01).And raw product group was better than the products group (P ＜0.01 or P ＜0.05),water extraction was better than powder group,from the different dose groups,high dose group was better than low dose group.Conclusion Cistanche before and after processing of normal rats had different degree of laxative action,which will provide a scientific basis for clinical rational use of Cistanche.%目的 研究肉苁蓉炮制前后对正常大鼠润肠通便作用.方法 将SD大鼠灌胃给予相对应的供试品溶液,观察大鼠胃肠激素和肠神经递质等指标,比较各组结肠病理切片变化情况以及结肠组织中P物质(SP)和血管活性肠肽(VIP)的分布和含量的变化.结果 与正常对照组比较,各治疗组血清中AchE显著升高(P＜0.01),ET-1、NO有下降的趋势.与正常对照组比较,各治疗组结肠组织中5-HT显著升高(P＜0.01,P＜0.05),AQP3显著下降(P＜0.01,P＜0.05).从结肠的病理切片图可以看出,生肉苁蓉水提高剂量组和阳性药对照组接近于正常组,其余治疗组比较,差异无统计学意义.对正常大鼠结肠组织中VIP、SP免疫组化定性表达的数据中可以发现,与正常对照组比较,各治疗组SP显著升高(P＜0.01),VIP有上升的趋势.与制品粉末低剂量组比较,生品水提组、生品粉末组SP、VIP显著增加(P ＜0.05,P＜0.01),且生品组均好于制品组(P＜0.01或P＜0.05),水提组好于粉末组,不同剂量组比较,高剂量组好于低剂量组.结论 肉苁蓉炮制前后对正常大鼠具有不同程度的润肠通便作用,其中生品的通便作用好于制品,旨在为临床合理使用肉苁蓉生制品提供一定依据.