苦豆碱对膀胱癌EJ细胞增殖与凋亡的影响

摘要

目的 观察苦豆碱对膀胱癌EJ细胞增殖与凋亡的影响并探讨其作用机制.方法 实验分组如下,A组:膀胱癌EJ细胞不作处理;B组:加入苦豆碱浓度25 μmol/L;C组:加入苦豆碱浓度50μmol/L;D组:加入苦豆碱浓度100 μmol/L.通过CCK8检测各组细胞的存活率,通过流式细胞术检测各组细胞凋亡率,通过Western blot法检测各组细胞Bcl-2、Bax、p-Erk1/2蛋白的表达水平.结果 CCK8结果显示,B、C、D组较A组细胞存活率降低(P＜0.05);流式细胞术结果显示:B、C、D组较A组细胞凋亡率增加(P＜ 0.05);Western blot结果显示:B、C 、D组较A组Bcl-2、p-Erk1/2表达水平降低(P＜0.05),Bax表达水平增高(P＜0.05).结论 苦豆碱可降低膀胱癌EJ的存活率,诱导其凋亡,其作用机制可能是通过抑制Erk1/2的磷酸化而实现的.%Objective To investigate the effect of aloperine on the proliferation and apoptosis of bladder cancer EJ cells and to explore its mechanism.Methods;The experimental group was as follows:bladder cancer EJ cells were not treated (group A);25μmol/L aloperine was added (group B);50μmol/L aloperine was added (group C).100μmol/L aloperine was added (group D).The survival rate of the cells was detected by CCK8.The apoptosis rate was detected by flow cytometry.The expression levels of Bcl-2,Bax,p Erk1/2 protein were detected by Western blot.Results CCK8 results showed that:B,C,D group compared with A group,the cell survival rate were decreased (P ＜ 0.05).The results of flow cytometry showed that the apoptosis rate of B,C and D group was higher than that of A group (P ＜ 0.05).Western blot results showed that:B,C,D group compared with A group the expression of Bcl-2 and p Erk1/2 levels were reduced (P ＜ 0.05),the expression of Bax level was increased (P ＜ 0.05).Conclusion The survival rate of EJ in bladder cancer can be reduced by aloperine and induce its apoptosis,and inhibiting the phosphorylation of Erk1/2 may be the mechanism.