血浆uPA、PAI-1和其4G/5G多态性与鼻咽癌临床分期关系

摘要

Objective To examine plasma concentrations of urokinasetype plasminogen activator (u-PA) and plasminogen activator inhibitor-1 (PAI-1),and a single nucleotide insertion/deletion (4G/5G) polymorphism located in the promoter region of PAI1 gene in normal subjects and the patients with nasopharyngeal carcinoma (NPC) at different stages.Methods Eighty six blood samples were collected from the patients with NPC,and 35 blood samples were taken from healthy people as control.Plasma concentrations of uPA and PAI-1 were measured by enzyme-linked immunosorbent assay (ELISA).DNA was extracted by micro extraction and the 4G/ 5Gpolymorphism wcrc analyzed by PCR using specific primers.Results An increase in u-PA antigen was observed with the advanced stages of the disease.However,the levels of PAI-1 antigen decreased with each advanced stage.Compared with control group,the 4G and 5G allele frequency located in the promoter region of PAI-1 gene was no significant difference (P ＞0.05) in the NPC group.Conclusion The plasma concentrations of uPA were increased while the PAI-1 concentrations were decreased in patients with NPC in stagesrelated manner.There is no correlation between the 4G/5G polymorphism and NPC.These results suggest that administration of antiplasminic agents may be effective in suppressing tumor invasion.%目的 探讨尿激酶型纤溶酶原激活物(uPA)、纤溶酶原激活物抑制剂1(PAI-1)的血浆浓度和PAI-1基因启动子区4G/5G多态性与鼻咽癌临床分期的相关性.方法 收集86例鼻咽癌患者血液标本,35例健康人血液标本作对照.所有标本均采用用酶联免疫吸附法(ELISA法)检测uPA和PAI-1的血浆浓度,微量法提取DNA,采用等位基因特异性引物PCR分析PAI-1基因启动子4G/5G多态性.结果 与正常对照组相比,鼻咽癌组血浆uPA浓度明显升高(P＜0.05),而血浆PAI-1浓度显著降低(P＜0.05),上述效应和临床分期具有显著相关性;PAI-1启动子区4G和5G基因频率与对照组相比差异无统计学意义(P＞0.05).结论 鼻咽癌的临床分期和恶性程度和血浆uPA浓度呈正相关,与PAI-1浓度呈负相关;PAI-1基因4G/5G 多态性与鼻咽癌没有相关性.