Objective To investigate the expression of human complement factor D (CFD) in the cervical squamous cell carcinoma and precancerous lesions in Uighur women and its association with clinicopathological outcomes. Methods We collected 72 cases of fresh tissues and 82 cases of paraffin-embedded tissue specimens of cervical squamous cell carcinoma ( CSCC) , cervical intraepi-thelial neoplasia ( CIN) Ⅱ-Ⅲ and cervicitis from Uighur women. After extraction of total RNA from fresh tissues, the mRNA expression of CFD gene in different cervical lesions was detected by semi -quantitative RT-PCR. The CFD protein expression level in cervical tissue specimens was measured by immunohistochemistry ( S-P method) . Results The mRNA expression level of CFD was re- markably decreased with the development of cervical lesion , with significant differences found in CFD expression between CIN Ⅱ-Ⅲ or CSCC and cervicitis ( P < 0. 05) . But no difference was not -ed between CIN Ⅱ-Ⅲ and CSCC ( P > 0. 05 ) . The immunohistochemistry showed a significantly lower expression of CFD protein in CSCC (22. 7% ) than in cervicitis (55. 6% , P <0. 05) and CIN Ⅱ-Ⅲ (55. 0% , P <0. 05) , but there was no statistical significance between CIN Ⅱ-Ⅲ and cervicitis (P>0. 05) . Conclusion The down-regulation of CFD at the transcriptional and translational level was closely associated with the development of cervical cancer in Uighur women , and CFD may become a molecular marker for early diagnosis of cervial cancer.%目的 利用维吾尔族妇女宫颈癌疾病资源,探讨宫颈鳞癌及癌前病变与补体因子D(complement factor D,CFD)表达调控的关系及临床意义.方法收集维吾尔族妇女宫颈鳞癌(cervical squamous cell carcinoma,CSCC)、宫颈内上皮瘤变(cervical intraepithelial neoplasia,CIN)Ⅱ-Ⅲ及宫颈炎患者的新鲜组织标本72例及石蜡包埋组织切片82例,提取新鲜组织RNA,利用半定量RT-PCR方法,鉴定不同宫颈病变组织中CFD mRNA表达水平;利用免疫组织化学(SP)法,研究不同宫颈病变组织切片中CFD蛋白质表达水平变化.结果 随着维吾尔族妇女宫颈病变进程,CFD mRNA表达水平明显下调,CINⅡ-Ⅲ或CSCC与宫颈炎相比,有显著差异(P＜0.05),但是CINⅡ-Ⅲ与CSCC之间无差异(P＞0.05).通过免疫组织化学鉴定,发现CSCC组织的CFD蛋白质表达水平(22.7 %)显著低于宫颈炎(55.6 %,P＜0.05)和CINⅡ-Ⅲ(50 %;P＜0.05),但CINⅡ-Ⅲ与宫颈炎的差异不显著(P＞0.05).结论 CFD转录表达下调或蛋白质表达水平下降与维族妇女宫颈癌发生有密切关系,可能成为肿瘤早期预警的分子标志物.
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