肝连续薄层冠状断面数据获取研究

摘要

目的 应用数控冷冻铣削技术获取肝的连续薄层冠状断面数据集.方法 经CT、MRI扫描筛选后,选择无明显病变的成年男性尸体1例,截取其上腹部,按直立位置于包埋容器内,经明胶包埋和深低温冷冻后,在低温冷冻室内用数控铣床从前向后连续铣削,获得肝的连续薄层冠状断面,用数码相机逐层拍摄,获取原始数据.结果 该例上腹部标本冷冻铣削后共获得层厚为0.2mm的肝连续冠状断面图像699幅,断面能够清晰显示肝的形态结构、位置及毗邻关系和肝内管道系统及其分支的走行分布.结论 应用数控冷冻铣削技术获取的肝连续薄层冠状断面数据集精度高,质量优,为基于冠状断面数据集的肝及肝内管道系统的三维重建奠定了基础.%Objective To obtain the thin coronal sectional anatomic dataset of the liver using digital freezing milling technique.Methods The upper abdomen of one Chinese adult cadaver was selected as the specimen.After CT and MRI examination verified no organic lesions,the specimen was embedded with gelatin in stand erect position and frozen under profound hypothermia,and then the specimen was serially sectioned from anterior to posterior layer by layer with digital cryomacrotome in the freezing chamber.The sequential images were captured by means of a digital camera and the dataset was imported to imaging workstation.Results The thin serial section of the liver added up 699 layers with each layer being 0.2 mm in thickness.The shape,location,structure,intrahepatic vessels,and adjacent structures of the liver were displayed clearly on each layer of the coronal sectional slice.Conclusion The thin coronal sectional anatomic dataset of the liver obtained by the digital freezing milling technique is of high precision and good quality.This provides the foundation for three dimensional reconstruction of the liver and intrahepatic vessels based on coronal sectional dataset of the liver.