目的:对呼吸道苛养菌的分离培养以及耐药性进行研究.方法:收集呼吸道感染患者的痰标本1200份进行分离培养, 通过纸片法对β-内酰胺酶产生率进行测定.结果:痰样本当中共分离出118株流感嗜血杆菌、64株副流感嗜血杆菌、207株肺炎链球菌.流感嗜血杆菌对磺胺甲恶唑、四环素以及氨苄西林等药物高度耐药;副流感嗜血杆菌对氧氟沙星、四环素以及氨苄西林具有较高的耐药性;肺炎链球菌对磺胺甲恶唑、四环素、克林霉素和红霉素表现出较高的耐药性, 对青霉素中介率达到10.14％.结论:对呼吸道苛养菌进行分离培养和药敏试验, 对合理应用抗生素、降低药物耐药性意义重大.%Objective: To study the isolation, cultivation and drug resistance of hypertrophic bacteria in respiratory tract. Methods: A total of 1200 sputum samples from patients with respiratory infections were collected and cultured. The rate of β-lactamase production was determined by the disk method. Results: A total of 118 strains of haemophilus influenzae, 64 strains of haemophilus parabens, and 207 strains of streptococcus pneumoniae were isolated from the sputum samples. Haemophilus influenzae is highly resistant to drugs such as sulfamethoxazole, tetracycline, and ampicillin; Hemophilus influenzae has high resistance to ofloxacin, tetracycline and ampicillin; Streptococcus pneumoniae showed high resistance to sulfamethoxazole, tetracycline, clindamycin and erythromycin, and the intermediate rate to penicillin reached 10.14％. Conclusion: The isolation, cultivation and drug susceptibility tests of respiratory bacteriophage are of great significance for rational application of antibiotics and reduction of drug resistance.
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