富贵籽愈伤组织诱导不定芽的研究

摘要

为了科学合理地开发利用具有观赏与药用价值的富贵籽,采用组织培养技术,考察不同植物激素组合的培养基对种子胚和茎段愈伤组织诱导以及愈伤组织诱导不定芽的影响。结果显示：（1）MS＋2,4-D 1.0＋BA 0.05的培养基配方较适合用于富贵籽种子胚诱导形成愈伤组织;（2）MS＋NAA 1.5 0＋BA 2.00的组合配方能较好的诱导富贵籽茎段产生愈伤组织;（3）MS＋6-BA 0.6＋NAA 0.05的培养基较适合于诱导富贵籽的原代愈伤组织分化产生不定芽。%In order to exploit and utilize the plant resource of Ardisia crenata(an ornamental and medicinal plant) scientifically and rationally,the research of adventitious buds induction from callus was carried out.According to the experience of the early research,single-factor experimental design,which used different auxin types and concentrations,was adopted for callus induction from seed embryo and stem of Ardisia crenata.The experiment,which selected the combination of NAA 0.05 m·L-1(the same as below) with different concentrations of BA,was conducted for adventitious buds induction from different subculture callus of Ardisia crenata.The results showed(1) The medium formula of MS + 2,4-D 1.0 + BA 0.05 was suitable for callus induction from seed embryo;(2) The medium formula of MS + NAA 1.5 0 + BA 2.00 was suitable for callus induction from stem;(3) The medium formula of MS + 6-BA 0.6 + NAA 0.05 was suitable for adventitious buds from primary callus.