门多萨假单胞菌DS04-T对PHBV降解性能研究

摘要

Using poly (3-hydroxybutyrateco-3-hydroxyvalerate) (PHBV) as sole carbon source ,the biodegradation of the PHBV by Pseudomonas mendocina DS04-T was studied .The effect of incubation time ,cultivation temperature ,initial pH , shaking revolution ,liquid medium volume and inoculation content on PHBV degradability were studied .According to the results of orthogonal test ,the optimal conditions of enzyme production were chosen as follows :temperature 28 ℃ ,incubation time 32 h ,initial pH 7 .5 ,shaking revolution 150 r/min ,inoculation content 1 .5% ,and 100 mL culture medium is load in 250 mL flask .Under the optimum conditions ,the PHBV-degrading enzymatic activity reached (18 .9 ± 1 .2) U/mL .%以3-羟基丁酸-co-3-羟基戊酸共聚物（PHBV）为唯一碳源培养PHBV降解菌DS04-T ，考察培养时间、温度、培养基起始pH、摇床转速、接种体积分数以及装液量等环境因素对菌株产生PHBV降解酶的影响，进而考察菌株对PHBV的降解能力。并结合正交试验优选适宜的产酶条件如下：培养温度28℃；培养时间32 h ；摇床转速150 r/min；装液量100 mL 培养基；培养基起始 pH 为7．5；接种体积分数1．5％。在此优化条件下菌株产生的PHBV降解酶有较高的活力，达（18．9±1．2） U/mL。