目的 研究中国（未包含香港、澳门和台湾地区）流式细胞术( FCM)急性白血病免疫分 型的应用现状，为制定FCM急性白血病免疫分型指南提供依据。方法检索中国医院知识仓库(CHKD)期刊全文库1994年至2009年公开发表的有关FCM急性白血病免疫分型方面的论文，并采用GraphPad Prism 4进行统计学分析。结果 中国FCM急性白血病免疫分型论文数呈逐年上升趋势，地区分布上逐渐由大城市到周边中小城市,论文数排在前3位的地区依次为北京、江苏和广东。在标本处理方面，采用单个核细胞标记和全血标记溶血洗涤者分别占34.2％( 113/330)和65.8％(217/330)。单色、双色、三色及四色荧光标记者分别占15.4％(53/344)、13.7％(47/344)、54.1％( 186/344)和16.3％(56/344)。荧光抗体数量从＜10个到＞20个不等，其中多数在11～ 16个，占48.6％(167/344)。在数据获取和分析方面，采用FSC/SSC和CD45/SSC设门者分别占32.6％(102/313)和67.4％(211/313)。淋系、髓系和胞质抗原表达的阳性判断标准尚不统一，淋系、髓系以≥20％为主，分别占64.8％(223/344)和95.4％(328/344)。胞质抗原以≥10％为主，占61.2％( 156/255)。结论中国FCM急性白血病免疫表型分析在标本处理、设门方法、数据获取及分析等方面均形成了共识。在抗体数量和组合、抗原阳性判定标准方面还存在一定的差异。%Objective To investigate the current situation of immunophenotyping of acute leukemia by flow cytometry in China (except Taiwan, Hongkong and Macau), and to provide theoretical evidence for the formulation of guidelines of immunophenotyping of leukemia by flow cytometry. Methods The Chinese Hospital Knowledge Database (CHKD) was used to retrieve the 1994-2009 published papers on immunophenotyping of acute leukemia by flow cytometry. All of 380 retrieved papers were analyzed by the GraphPad Prism 4. Results The number of published papers in China increased yearly and changed gradually from large cities to small and medium-sized cities in local distribution. The most often reported area was Beijing, followed by Jiangsu and Guangdong. In specimen processing, the ratios of using mononuclear cell labling method and whole blood labling-lysing-washing method were 34.2 ％ (113/330) and 65.8 ％ (217/330) respectively. The ratios of using single color, two color, three color and four color labling method were 15.4 ％ (53/344), 13.7 ％ (47/344), 54.1％ (186/344) and 16.3 ％ (56/344), respectively. The number of fluorescent antibodies was used from less than 10 to more than 20. Most of them were between 11 to 16, which was 48.6 ％ (167/344) of total number of published papers. In data acquisition and analysis, the ratios of using FSC/SSC gating and CD45/SSC gating were 34.2 ％ (113/330) and 65.8 ％ (217/330), respectively. There were some differences in the positive criteria of lymphatic and myeloid and cytoplasm antigens. The main positive criteria of lymphatic, myeloid antigen was ≥20 ％, which was 64.8 ％ (223/344) and 95.4 ％ (328/344) . The main positive criteria of cytoplasm antigen was ≥ 10 ％, which accounted for 61.2 ％ (156/255). Conclusion A common view has been reached in specimen processing, gating method, data acquisition and analysis in immunophenotyping of acute leukemia by flow cytometry. But there were still some difference in the number of fluorescent antibodies, panels and evaluation of positive criteria of antigens.
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