Objective To determine the expression of indole-2,3 dioxygenase (IDO) in human acute leukemia,and to investigate its correlations with clinicopathological parameters and prognosis in acute leukemia.Methods The expression of IDO in protein and RNA levels was detected by immunohistochemistry and real-time quantitative RT-PCR,respectively,and the correlations of IDO with clinicopathologic features and prognosis of acute myeloid leukemia (AML)-M5 were analyzed.Results The positive rate of IDO protein was 63.3 ％ (38/60) in human acute leukemia,while it in AML (34/49,69.4 ％),especially in AML-M5 patients (29/35,82.9 ％),was significantly higher than that of acute lymphoblastic leukemia (4/11,36.4 ％).The expression of IDO protein in healthy human peripheral blood mononuclear cells was negative.The RNA expression level of IDO in AML-M5 or non AML-M5 patients were significantly higher than that of healthy people (P ＜ 0.001),and AML-M5 patients had significantly higher IDO RNA level than that in non AML-M5 patients (P ＜ 0.05).The IDO gene expression was not correlated with sex,age and drug sensitivity,while it was closely related with these factors in the patients without complication of pulmonary infection.IDO could not act as an independent prognostic marker.Conclusion The expression of IDO in AML-M5 patients is significantly higher than that in non AML-M5 patients and healthy people.The positive expression of IDO is associated with poor prognosis of AML-M5 patients,but it is not an independent poor prognostic indicator.%目的 探讨急性白血病细胞吲哚胺2,3双加氧酶(IDO)的表达与急性白血病临床病理参数和预后的关系.方法 分别采用免疫组织化学法及实时荧光定量反转录PCR法检测60例急性白血病患者白血病细胞IDO蛋白和RNA表达水平,并与临床资料相结合进行分析.结果 急性白血病患者IDO蛋白阳性表达率为63.3％(38/60),其中急性髓系白血病(AML)患者的阳性率为69.4％(34/49),尤其是AML-M5型患者阳性率达82.9％(29/35);而急性淋巴细胞白血病患者为36.4％(4/11).IDO蛋白在健康人外周血单个核细胞呈阴性表达.IDO RNA在AML-M5组的表达水平高于非AML-M5组(P＜0.05)和健康人组(P＜ 0.001),非AML-M5组亦高于健康人(P＜ 0.001).IDO RNA的表达水平与患者的性别、年龄、药物敏感性不相关,与患者是否合并肺部感染相关.IDO阳性表达者有较差的预后,但IDO并不是AML-M5患者独立的预后不良指标.结论 IDO在AML-M5患者的表达较非AML-M5患者和健康人明显升高.IDO的阳性表达影响AML-M5患者的预后,但不是独立预后不良指标.
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