活化诱导胞嘧啶核苷脱氨酶基因在白血病中的表达

摘要

Objective To investigate the characteristics of activation-induced cytidine deaminase (AID) expression level in de novo acute leukemia (AL) patients, chronic myeloid leukemia chronic phase (CML-CP), chronic myeloid leukemia blastic crisis (CML-BC) patients and leukemia cell lines. Methods The expression level of AID mRNA was measured in 89 cases of newly-diagnosed acute lymphoblastic leukemia (ALL) patients, 79 cases of de novo acute myeloid leukemia (AML) patients, 5 cases of CML-BC patients, 5 cases of CML-CP patients and leukemia cell lines NB4, THP-1, KG-1, Raji, K562 by real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR), bone marrow mononuclear cells of 16 normal healthy donors were used as the control group. Results The expression levels of AID mRNA in 89 cases of ALL and 79 cases of AML were 0.006-7 463.175 and 0.005-69.107, the median expression levels were 3.785 and 1.812, the expression level of AID mRNA in the normal control group was 0.146-4.707, and the median expression level was 1.483, respectively. The AID expression levels of ALL, B-ALL, Burkitt leukemia, M4 patients and Raji cells were significantly higher than those of the normal control group (all P <0.05). Nevertheless, the AID mRNA expression levels of M3 patients and NB4, KG-1 cells were lower than those of the normal control group (all P <0.05). Furthermore, the AID mRNA expression levels of K562 cell were strikingly higher than that of the CML-CP patients (P<0.001), so were those of CML-BC, chronic myeloid leukemia myeloid blast crisis (CML-MBC), chronic myeloid leukemia lymphoblastic blast crisis (CML-LBC) patients. Conclusion AID gene shows high expression level in B-ALL, Burkitt leukemia and M4, low expression level in M3 and KG-1 cells, and obvious high expression level in CML-BC.%目的：探讨活化诱导胞嘧啶核苷脱氨酶（AID）在急性白血病（AL）、慢性粒细胞白血病慢性期（CML-CP）和急变期（CML-BC）患者及白血病细胞株中的表达特点。方法采用实时定量反转录聚合酶链反应（RT-PCR）检测89例初诊急性淋巴细胞白血病（ALL）患者、79例初诊急性髓系白血病（AML）患者、5例 CML-BC 患者、5例 CML-CP 患者和白血病细胞株 NB4、THP-1、KG-1、Raji、K562中 AID mRNA 的表达，以16名健康供者骨髓单个核细胞作为对照。结果89例 ALL 患者和79例 AML 患者 AID mRNA的中位表达水平分别为3.785（0.006～7463.175）、1.812（0.005～69.107），健康对照组 AID mRNA 的表达水平为1.483（0.146～4.707）。 ALL、B-ALL、伯基特型白血病、Raji 细胞株和 M4的 AID mRNA 表达水平较健康对照组升高，差异均有统计学意义（均 P＜0.05），而 M3、NB4和 KG-1细胞株的 AID mRNA 表达水平较健康对照组下降，差异均有统计学意义（均 P＜0.05）。 K562细胞株的 AID mRNA 表达水平较 CML-CP患者升高，差异有统计学意义（P＜0.001）。 CML-BC、CML 淋系急性变（CML-LBC）和 CML 髓系急性变（CML-MBC）患者的 AID mRNA 表达水平也高于 CML-CP 患者。结论 AID 在 B-ALL、伯基特型白血病、M4中呈高水平，在 M3和 KG-1细胞株中呈低表达，在 CML-BC 中出现表达水平的显著升高。