重组人促红细胞生成素后处理对兔缺血/再灌注骨骼肌细胞凋亡及超微结构的影响

摘要

Objective To observe the effects of recombinant human erythropoietin (rhEPO) postconditioning on apoptosis and ultrastructure from ischemia/reperfusion (I/R) injury in rabbitsskeletal muscle.Methods 24 adult rabbits were randomly divided into 3 groups, namely, blankcontrol group, I/R group and rhEPO postconditioning group (5000 IU/kg).With 25％ utethane (4 mL/kg) ear vein injection of anesthesia, I/R models of the left hind limbs of rabbitswere set up in I/R group and rhEPO postconditioning group.After 4 hours the arteries wereopened and the blood flow perfusion was restored, after 12 hours the gastrocnemius samples weretaken out to detect apoptosis in three groups by using terminal deoxynucleotidyl transferasemediated deoxy-uridine triphosphate nick end labeling and to make light and electronic microscopic observation.Results Compared with I/R group, the apoptosis of rhEPO postconditioning group reduced significantly (P ＜0.05), skeletal muscle injury were seen in both I/R group andrhEPO postconditioning group under light and electronic microscope, but the damage in rhEPO postconditioning group was significantly lighter than that in I/R group.Conclusion rhEPO postconditioning can reduce apoptosis of skeletal muscle cell and improve I/R injury.%目的 探讨重组人促红细胞生成素(rhEPO)后处理对家兔缺血/再灌注(I/R)骨骼肌细胞凋亡及超微结构的影响.方法 将24只成年家兔随机分为对照组、I/R组和rhEPO后处理组(5000 IU/kg),采用25%的乌拉坦4 mL/kg耳缘静脉注射麻醉,在I/R组、rhEPO后处理组建立兔左后肢腓肠肌I/R实验模型,4h后松开动脉夹,恢复血流灌注,12 h后切取腓肠肌标本,采用原位末端标记法检测3组细胞凋亡指数,对I/R早期骨骼肌病理标本进行光镜及电镜观察.结果 rhEPO后处理组细胞凋亡较I/R组明显减少,差异有统计学意义(P＜0.05);电镜及光镜下腓肠肌组织病理改变显示,rhEPO后处理组病变程度明显轻于I/R组.结论 rhEPO后处理可通过减少细胞凋亡,改善骨骼肌I/R损伤.