旋覆花中两种倍半萜内酯提取方法研究

摘要

Three methods of ultrasonic,reflux and microwave were used to study the best extracting method and condition.The influ-ence of ultrasonic was investigated through single factor test and orthogonal design test to get the best extract conditions:optimized pa-rameters of ethanol/water (70∶30,v/v),45 min extract time and 1∶15 of solute to solvent ratio.The result showed that the best condi-tion established by orthogonal design test was proved to be practical.%采用超声、回流和微波3种方法，探讨提取旋覆花中两种倍半萜内酯的最佳提取方法和条件，并通过单因素实验和正交设计方法研究超声提取的影响因素，确定最佳提取条件为：乙醇溶液体积分数70％，提取时间45 min，料液比（g／mL）1∶15．验证实验结果表明，正交实验所得最佳条件切实可取．