目的探讨利用rhHSP70联合Mage-3抗原肽修饰树突状细胞递呈肿瘤抗原的特性增强抗乳腺癌的作用．方法利用GM-CSF和IL-4诱导外周血单个核细胞产生树突状细胞，负载Mage-3抗原肽的同时加入新型热休克蛋白（Hsp70L1），不同分组分别诱导自体CTLs产生．ELISA测定细胞因子的分泌和CTLs杀伤活性．结果Mage-3抗原肽致敏的DCs增加CTLs增殖，上调细胞因子TNF-α、IL-6的分泌；Mage-3抗原肽致敏的DCs诱导的CTLs淋巴细胞对人乳腺癌细胞MCF-7有明显杀伤效应，并以rhHSP70联合Mage-3抗原肽组最为明显，能显著上调细胞因子TNF-α、IL-6的分泌与增强CTLs对肿瘤细胞的杀伤率．结论 rhHSP70联合Mage-3抗原修饰的DCS生物活性增强，能刺激淋巴细胞的增殖，使细胞因子的分泌量提高．rhHSP70联合Mage-3抗原修饰的DCS诱导的CTLs杀乳腺癌细胞作用明显增强．%Objective To make use of the characteristics of presenting and processing tumor antigen of Dendritic cells pulsed with recombinant human Hsp70 and Mage-3 peptide to enhance killing capability of breast neoplasms. Methods Autologous dendritic cells were isolated from PBMC and then stimuiated in vitro with GM-CSF and IL-4.Dendritic cells were loaded with Mage-3 peptide;at the same time Hsp70L1 was added. The specific groupings each induce generation of tumor specific cytotoxic assay with ELISA. Results DCs pulsed with Mage-3 peptide enhanced the growth expansion of CTL, and promoted the secretion of cytokines such as TNF-αand IL-6. DCs pulsed with recombinant human Hsp70 and Mage-3 peptide had cytotoxicity against human breast cancer cells MCF-7.Conclusions DCs pulsed with recombinant human Hsp70 and Mage -3 peptide have higher biological activities. Modified DCs can stimulate the proliferation of lymphoeytes,present effectively tumor antigen to stimulate generation of speeific CTLs. Dendritic cells pulsed with recombinant human Hsp70 and Mage-3 peptide have high ability to kill breast cancer cells.
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