目的 探讨Cytohesin-2在肝癌细胞株中的表达情况以及对肝癌细胞增殖的影响.方法 应用western blot法检测6种细胞株中Cytohesin-2的表达情况,筛选出高表达细胞株后,给予Cytohesin-2的特异性抑制剂SecinH3了解Cytohesin-2对Hep3B细胞增殖的影响.结果 Cytohesin-2蛋白在Hep3B、HepG2、SMCC-7721、BEL-7402、Huh-7中的表达均高于人正常肝细胞系HL-7702中的表达.应用Cytohesin-2抑制剂SecinH3后,Hep3B细胞的增殖能力受到显著抑制,并且Hep3B细胞的凋亡数量明显增加.结论 Cytohesin-2在多种肝癌细胞系中均具有显著地高表达,并可以通过抑制Hep3B细胞凋亡的方式促进Hep3B细胞的增殖.Cytohesin-2可能作为肝细胞肝癌生物治疗的新的靶点.%Objective To investigate the expression of Cytohesin-2 protein in hepatoma cells and its role in proliferation of hepatoma cells. Methods Cytohesin-2 protein expression was examined by western blot. Cytohesin-2 antagonist SecinH3 was used to Hep3B, and proliferation of hep3B was detected by MTT and flowcytometry. Results The expression levels of Cytohesin-2 were higher in Hep3B, HepG2, SMCC-7721, BEL-7402 and Huh-7 than those in HL-7702. And the proliferation of Hep3B cells was significantly inhibited after treatment with SecinH3, while the apoptosis of was increased in Hep3B cells. Conclusion Cytohesin-2 has extremly high expression in hepatoma cells, and it can promote proliferation and inhibit apoptosis of Hep3B cell. It should be a novel target for biotherapy of hepatocellular carcinoma in the future
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