小麦淀粉合成酶基因实时荧光定量PCR 检测方法的建立及其应用

摘要

Starch synthase genes (AGPase 1、GBSSI 、SSSIII、SBEI)in wheat grains is quanti-tatively detected by rea1-time fluorescent quantitative PCR. The four starch synthase genes and endogenous gene(18s)are amplified respectively. The standard curve of Ct value amplified by the AGPase1、GBSSI、SSSIII、SBEIgenes or 18s gene vs amounts of the DNA template is generated and a linear regression equation is obtained consequently. The expressions of AGPase1、GBSSI、SSSIII、SBEI during the wheat grouting are quantitively analyzed by the established method.%利用荧光定量PCR技术，对小麦淀粉合成酶基因(AGPase1、GBSSI、SSSIII、SBEI)进行了定量检测，分别建立了这4种淀粉合成酶基因和内参基因18s的Ct值与模板量之间的标准曲线和线性回归方程。运用所建立的方法对小麦籽粒4种淀粉合成酶基因在灌浆期间的表达变化情况进行了定量分析。