目的：探讨α-干扰素(α-IFN)、异搏定(VRP)对RCC-925肾癌细胞阿霉素敏感性的影响。方法：(1)将RCC-925肾癌细胞分别与不同浓度的逆转剂α-干扰素或异搏定或α-干扰素+异搏定及与不同浓度阿霉素(ADM)共同培养；MTT法检测肿瘤细胞的存活率。(2)图像分析仪检测逆转前后PgP(P-glycoprotein)表达。结果：单独应用α-干扰素无逆转作用；联合应用α-干扰素、异搏定显著逆转肿瘤细胞的耐药性，效果优于单用异搏定(P＜0．05)；2．5 mg/L异搏定+500pg/mLα-干扰素共同与细胞作用后，PgP表达降低。结论：α-干扰素、异搏定作为逆转剂联合使用，能显著逆转RCC-925细胞的耐药性，可望为临床耐药肿瘤的治疗提供一种新的方法。%Aim: RCC-925 is a cell line of human renal cell carcinoma, effect of α-interferon (α-IFN) and verapamil(VRP) on adrianmycin(ADM) in RCC-925 cells. Methords: (1) RCC-925 cells were culture in vitro with different concentration of α-IFN or VRP or α-IFN+VRP and 1 mg/L ADM, or with the different concentration of ADM and 2.5 mg/L VRP or 500 pg/mL α-IFN or 2.5mg/L VRP + 500 pg/mL α-IFN, living cells were detected by MTT colorimetric assay. (2) The expression of PgP RCC-925 cells was conducted by immunohischemical techniques was used for α-IFN and VRP. Results: The cytotoxicity studies showed that sole use of α-interferon didn't show its reversal function while verapamil combinated with α-interferon was significantly more effective than the sole use of verapamil in overcoming drug resistance (P＜0.05). The expression of P - glycoprotein studies demonstrated the low-level expression in culturing with 500 pg/mL α-interferion and 2.5 mg/L verapamil. Conclusion: The results have indicated that multidrug resistance could be significant reversed by verapamil combinating with α-interferon, would provide a novel therapy in the treatment of cancer patients.
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