RP-HPLC法测定火炭母药材中鞣花酸的质量分数

摘要

采用色谱柱为Welch Materials XB-C18柱(4.6 mm×250 mm,3μm),流动相为V甲醇:V体积分数为0.2%的甲酸溶液=45∶55,流速为0.8 mL·min-1,柱温为30℃,检测波长为357nm.结果显示,鞣花酸对照品的峰面积与进样量在0.067～2.68 μg的范围内呈良好的线性关系(r=0.9999),该方法的平均回收率为99.6%,RSD为1.09%,测得10份样品中鞣花酸的质量分数在03592～3.439 mg·g-1范围内.本方法快速、简便、重复性好,可用于火炭母药材中鞣花酸的含量测定.%Ellagic acid in Polygonum chinense L. was determined by a RP-HPLC method using s XBC18 eolunm (4.6 mm ×250 mm, 3 μm). The mobile phase consisted of methanol and 0.2％ formic acid at a flow rate of 0. 8 mL/min. The detection wavelength was set at 357 nm and the column temperature was 30 ℃. The results show that the linear range of ellagic acid was 0.067 ～ 2.68 μg ( r =0.9999). The average recovery was 99.6 ％ ( RSD = 1.09 ％ ), and the content of ellagic acid in ten samples was in the range of 0.592 ～ 3.439 nag · g- 1. This method is found to be quick, simple and reproducible, and it is quite suitable to be used for the determination of ellagic acid in Polygonum chinense L.