VYSOKOCHUSTVITELNY METHOD FOR DETERMINING THE NUMBER components obtained from medicinal herbs

摘要

1. High-sensitivity method for determining the amounts of the components obtained from herbs, comprising: extraction step wherein an extract containing components obtained from herbs is prepared by: introducing the biological sample mixture with an alkali or an alcohol in the solid phase having the reverse phase distribution function and the anion exchange; washing the solid phase at least once with the cleaning liquid; and subsequent elution from the solid phase acidic alcohol, and the step of quantitative determination, in which at least one component selected from the group consisting of glycyrrhizin, glycyrrhetinic acid, metabolites of glycyrrhizin and glycyrrhetinic acid congeners glycyrrhizin and glycyrrhetinic acid components saponin contained in glycyrrhiza (licorice), and pharmacologically acceptable salts thereof, contained in the extract obtained in the extraction step, is detected and quantified by mass with pektrometrii; wherein the cleaning liquid is a single-component liquid or a liquid mixture of at least two components selected from the group consisting of water, alkali, alcohol and acetonitrile apos extraction step the solid phase is washed with a liquid mixture of alkali, alcohol and water, the liquid mixture of alkali and water or water, followed by washing with alcohol, a liquid mixture of alcohol and water or atsetonitrilom.2. Highly sensitive method for determining the amount of components derived from medicinal herbs according to Claim. 1, wherein in the extraction step the solid phase is washed with a liquid mixture of alkali, alcohol and water followed by washing with sleep