The Pleurotus ostreatus were used as the raw material for extracting polysaccharide by means of smashing extractor, ultrasonic extraction, ultrasonic-assisted complex enzyme extraction. The results indicate the optimal extraction conditions of the smashing extractor were extraction temperature 90 X^ , extraction rate 11 350 r/min, extraction time 20 min, extraction 3 times, and the yield of polysaccharide extracted was 11.20%. The optimal extraction conditions of ultrasonic extraction were extraction temperature 60 X, , extraction time 60 min, ultrasonic power 450 W, extraction 3 times, and the yield of polysaccharide extracted was 9. 75% . The optimal extraction conditions of ultrasonic-assisted complex enzyme extraction were extraction temperature 30 X. , extraction time 60 min, ultrasonic power 350 W, extraction pH 5, the yield of polysaccharide extracted was 12.05%. In comprehensive consideration of the yield of polysaccharide extracted, saving energy, simple operation, the smashing extractor was considered as an ideal method.%以平菇为原料,分别采用破碎、超声波和超声辅助复合酶3种提取方法提取平菇水溶性多糖.正交优化结果表明:提取温度90 ℃,剪切速率11 350 r/min,提取时间20 min,提取3次,破碎法提取多糖得率为11.20%; 提取温度60 ℃,超声功率450 W,提取时间60 min,提取3次,超声法提取多糖得率为9.75%; 提取温度30 ℃,超声功率350 W,提取时间60 min,溶液pH=5,超声辅助复合酶法提取多糖得率为12.05%.综合考虑提取得率、节能环保、操作方法等因素,选用破碎法提取平菇多糖较理想.
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