石油污染地下水中苯降解菌的代谢特征

摘要

从东北某油区石油污染的地下水中分离、纯化出苯降解优势菌B6,研究其降解效率和代谢特征,结果表明:当模拟石油污染地下水中苯的质量浓度为394.36 mg/L时,3 d后的降解率为82.82％；相关酶活及代谢产物在双加氧酶的作用下,B6菌降解苯的途径为儿茶酚正位裂解；B6菌在代谢过程中产生色氨酸酶、脂肪酶、淀粉酶、明胶酶、乳糖酶和氧化酶等,葡萄糖、乳糖、蛋白质、色氨酸、脂肪、淀粉、明胶、多肽、纤维素、含硫有机物和细胞色素C等为其营养物质,以亚硝酸盐和硝酸盐为电子受体；B6菌为革兰氏阳性红平红球菌.%The authors investigated the degradation features of benzene by strain B6 dominant strain isolated and purified from the petroleum-contaminated groundwater in the northeast of China. Results show that when the initial mass concentration of benzene was 394. 36 mg/L, the degradation rate of benzene by train B6 was 82. 82% on the third day; ortho 2 cleavage of catechol was the right approach of benzene degradation under the effect of dioxygenase; B6 strain could produce tryptophanase, lipase, amylases, gelatinase, lactase, oxidase, use glucose, lactose, protein, tryptophan, fat, starch, gelatine, polypeptide, cellulose, sulfur-containing organic compounds, cytochrome C as nutrients, and utilize nitrites and nitrates as electron acceptors in its metabolic process. It was identified that B6 strain was Rhodococcus erythropolis, gram-positive bacterium.