X射线对小鼠腹腔巨噬细胞表达B7-1和B7-2的影响

摘要

目的:观察X射线全身照射对小鼠腹腔巨噬细胞表达B7-1和B7-2的影响。方法:采用免疫组织化学法（ABC法）。结果:小鼠腹腔巨噬细胞表达B7-2达到高峰的时间早于B7-1，而且B7-2表达幅度也高于B7 -1。剂量-效应关系的研究表明, B7-1 和B7-2的表达均在0.07 5 Gy剂量点出现峰值，而在较大剂量照射时也出现一个峰值。但在不同分度分析结果中发现 ，低剂量X射线照射组的高密度细胞百分数比较大剂量组多。结论: B7分子的表达上调可能是参与低剂量电离辐射免疫增强效应的重要因素。%Objective: The effect of whole body irradiation (WBI) on B7-1 and B7-2 express ion in peritoneal macrophages in mice was observed.Methods:Immunohistochemistry (ABC method) was used. Results: The upregulation of B7-2 expression induced by WBI X-rays appeared ea r lier than that of B7-1.The study on the dose-effect relationship showed that t he ex pression of both B7-1 and B7-2 reached its peak at 0.075 Gy after WBI X-rays. Th e doses inducing a second peak of B7-1 and B7-2 upregulation were higher in th e case of WBI in comparison with in vitro irradiation. Conclusion: The results suggested that the upregulation of expression of B7 mole cules after irradiation might be an important factor involved in the enhancing e ffect of low dose radiation on immunity.