CC趋化因子受体6在人唾液腺腺样囊性癌组织中的表达及其临床意义

摘要

目的:探讨CC趋化因子受体6(CCR6)在人唾液腺腺样囊性癌组织中的表达,阐明其在人唾液腺腺样囊性癌神经侵袭中的作用机制.方法:利用免疫组织化学染色(SP法)检测30例唾液腺腺样囊性癌患者癌组织(根据癌细胞是否沿神经束周围或神经束间浸润生长,分为神经侵袭组18例,非神经侵袭组12例)及22例正常人唾液腺组织中CCR6的表达情况,分析癌组织中CCR6阳性表达率与患者临床病理特征的关系.结果:在30例唾液腺腺样囊性癌组织中22例CCR6呈阳性表达,阳性表达率为73.33％,免疫组织化学染色显示CCR6主要表达于癌细胞的细胞膜和细胞质中;在22例正常唾液腺组织中CCR6均呈阴性表达,2组CCR6阳性表达率比较差异有统计学意义(P＜0.05).其中,神经侵袭组和非神经侵袭组唾液腺腺样囊性癌患者癌组织中CCR6阳性表达率分别为94.44％ (17/18)和41.67％ (5/12),组间比较差异有统计学意义(P＜0.05);在不同年龄、性别和病理分型的唾液腺腺样囊性癌患者癌组织中CCR6的阳性表达率比较差异均无统计学意义(P＞0.05).结论:CCR6在人唾液腺腺样囊性癌的发生发展过程中起重要作用,且与其神经侵袭有密切关联.%Objective:To explore the expression of CC chemokine receptor 6 (CCR6) in human adenoid cystic carcinoma tissue of salivary glands,and to clarify the mechanism of CCR6 in the nerve invasion of human adenoid cystic carcinoma.Methods:Immumohistochemical method (SP) was performed to detect the expression of CCR6 in 30 cases of cancer tissue of the patients with adenoid cystic carcinoma (including 18 cases in nerve invasion group and 12 cases in non-nerve invasion group) and 22 cases of normal salivary gland tissue.The relationship between the positive expression rate of CCR6 and the clinicopathological features of the patients were analyzed.Results:CCR6was positively expressed in 22 cases among 30 cases of salivary adenoid cystic carcinoma tissue,and the positive expression rate was 73.33％.The immunohistochemical staining showed that CCR6 mainly expressed in the cell membrane and cytoplasm of cancer cells.All of the 22 cases of normal salivary gland tissue were negative to CCR6expression;the difference of the positive expression rate of CCR6 between two groups was statistically significant (P＜0.05).The positive expression rate in nerve invasion group and non-nerve invasion group were 94.44％(17/18) and 41.67％ (5/12),respectively;there was statistically significant between two groups (P＜0.05).The positive rates of CCR6 in the patients with salivary gland adenoid cystic carcinoma with different ages,sex and pathological types had no statistically significant differences (P＞0.05).Conclusion:CCR6 plays an important role in the development of human salivary adenoid cystic carcinoma and is closely related to the nerve invasion.