小刺猴头液体深层发酵浸膏多糖提取工艺的优化及分离纯化

摘要

Warm water extraction was used for Hericium caput-medusae liquid submerged fermentation ex-tractum polysaccharides, based on solvent-solid ratio, extracting time, extracting temperature single-factor experiments. The SAS 9.2 software was employed to design response surface and the yield of polysaccharides was used as the responsive values. The optimum ultrasonie-assisted extraction conditions were as follows: solvent/solid ratio 2.29= 1 (mL/g) , extracting time 4.52 h, extracting temperature 79.51, the yield of polysaccharides rate was 12.28 %. Ethyl alcohol fractionation, purified through deproteiniza-tion, dialysis and freeze-dry. The polysaccharides were identified as uniform after film electrophoresis,column chromatography, the spectrum of ultraviolet spectra and repeat freeze thawing.%采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29:1,提取时间4.52 h,提取温度79.5℃,2次平行多糖得率12.28%.通过乙醇分级、脱蛋白、透析、过凝胶柱分离多糖,采用醋酸纤维素薄膜电泳、柱层析、紫外光谱及反复冻融鉴定为均一多糖.