The embryos of three major maize inbred lines Dan988,Qi319 and Zheng58 were selected as gene receptors to explore the effects of genotype,size of embryo,concentration of 2,4-D and concentration of AgNO3 on the induction rate of embryonic callus by L9(34) designed orthogonal experiment.The results showed that the effects of each factor on the embryonic callus induction rate was genotype＞ embryo size ＞ 2,4-D concentration ＞ AgNO3 concentration and the optimum conditions were maize inbred line Qi319,embryo size 1.5-2.0 mm,2,4-D concentration 2 mg/L and AgNO3 concentration 8 mg/L,under which the embryo callus induction rate could be as high as 60.58％.The plant overexpression vector PCAMBIA3301-ICE-Bar was transferred into maize inbred line Qi319 by agrobacterium and 5 T0 transgenic positive plants were obtained through the preliminary screening by herbicide basta and the followed detection by PCR.%选取3个骨干玉米自交系丹988、齐319、郑58幼胚为材料,利用正交试验设计L9(34),研究了基因型、幼胚大小、2,4-D浓度和AgNO3浓度4个因素对胚性愈伤诱导率的影响.结果表明:各因素对愈伤诱导结果的影响大小依次为基因型＞幼胚大小＞2,4-D浓度＞AgN03浓度,最优组合为“齐319”、幼胚为1.5～2.0 mm、2,4-D浓度为2 mg/L和AgNO3浓度为8 mg/L,愈伤组织诱导率为60.58％.用农杆菌介导法将植物超表达载体pCAMBIA3301-ICE1-Bar转入玉米自交系“齐319”中,得到的转化植株经除草剂basta初步筛选后,通过PCR检测,获得了5棵T0代阳性植株.
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