结合农艺性状和SSR标记构建吉林省花生初级核心种质

摘要

Using 260 peanut test materials,the primary peanut core germplasm in Jilin province was constructed by different genetic distances.In 50％ sampling ratio,feasibility and validity of constructing primary core germplasm with different methods were evaluated by using quantitative trait parameters including mean difference percentage,variance difference percentage,range coincidence rate and variation ratio of variation coefficient.Finally,the appropriate method was used to construct the primary peanut core germplasm.The related parameters of SSR markers were used to verify the above results,which included allelic variance,effective number of allelic variation,Shannon-Weaver index,Nei expected heterozygosity,etc.The results showed that Euclidean distance,the preferred sampling method and the shortest distance method were applied in cluster analysis,and the peanut core germplasm of 128 accessions was constructed.Via the validation of the related parameters of SSR markers,128 accessions represented 80％ genetics information of the primary germplasm and could very well represent the genetic diversity of the original germplasm.%以260份花生材料为试材,采用不同的遗传距离构建吉林省花生初级核心种质.在50％的取样比例下,采用数量性状参数均值差异百分率、方差差异百分率、极差符合率、变异系数变化率4个指标评价不同方法构建的初级核心种质的可行性和有效性.最终选出一种合适的方法构建花生初级核心种质,并利用等位变异数、有效等位变异数、Shannon-Weaver指数、Nei期望杂合度等SSR标记相关参数进行验证.研究结果表明:基于欧式距离,采用优先取样法结合最短距离法进行聚类分析,构建包含128个样品的花生核心种质.采用SSR标记参数进行验证,表明128份初级核心种质可以代表原种质80％的遗传信息,较好地代表了原种质的遗传多样性.