以红桤木扦插苗为研究材料，进行放线菌结瘤处理并喷施无氮Hoagland营养液（T1），以及不结瘤处理但喷施硝态氮Hoagland营养液（T2）、无氮Hoagland营养液（T3）和混合氮Hoagland营养液（T4）等试验，比较研究了结瘤处理和氮形态对红桤木生长和生理指标的影响。结果表明，放线菌结瘤处理对红桤木生长和生物量累积具有显著的促进效果，对叶绿素含量及其组成具有明显的促进和调节效果。红桤木接种放线菌，使硝酸还原酶活性和谷氨酰胺合成酶活性维持在较高水平，过氧化物酶活性维持在最低水平。氮形态对红桤木生理指标及生物量也有比较强的效用。%The cutting seedlings of Alnus rubra were treated by nodulation ＋ non-nitrogen Hoagland culture solution, non- nodulation ＋ nitrate-nitrogen Hoagland solution, non-nodulation ＋ non-nitrogen Hoagland solution, and non-nodulation ＋ mixed-nitrogen Hoagland solution respectively, to show the different effects between nodulation and non-nodulation, and be- tween nitrate- and mixed-nitrogen on physiological characteristics and biomass of Alnus rubra. The results indicated that nodulation markedly improved the growth and biomass,increased the chlorophyll content and adjusted the chlorophyll con- stituents. After nodulation,A, rubra maintained fairly high activities of nitrate reductase and glutamine synthetase, but the lowest activity of peroxide enzyme. Nitrogen forms also had remarkable effects on the physiological characteristics and bio- mass.
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