选择8年生乌桕优树母株，取其枝条的腋芽茎段为外植体，进行组织培养快速繁殖技术研究。结果表明，接种时，先用70％的酒精对带芽茎段消毒30s，再用0．1％升汞灭菌11min，污染率可降到18．5％。在初代培养中，芽诱导最佳培养基为WPM＋6-BA1．5mg／L＋NAA0．2mg／L，30d后的诱导率可达85．4％。在4／5MS＋6-BA1．5mg／L＋KT0．5mg／L＋NAA0．05mg／L培养基中继代增殖培养，增殖效果最好，增殖倍数为3．8，生长高度达4．8cm。将健壮的试管芽苗接种于1／2MS＋IBA0．5mg／L＋NAA0．3mg／L＋Citricacid100mg／L培养基上进行生根诱导，生根率可达95．8％。移栽35d后，成活率达87．8％。%The stem sections with axillary bud of Sapium sebiferum （ L. ） Roxb. （ 8 years old ） were used as explants for the research of tissue culture and rapid propagation. The results showed that the contamination rate was 18.5% when we steri- lized the explants with ethanol（70% ） 30 seconds then used HgC1 z （0. 1% ） to sterilize the stem sections 11 minutes. The medium suitable for induction in primary culture was WPM ＋ 6 - BA 1.5 mg/L ＋ NAA 0.2 rag/L, and the inductivity reached to 85.4% after 30 days. The medium suitable for secondary proliferation was 4/5 MS ＋ 6 - BA 1.5 mg/L ＋ KT 0. 5 mg/L ＋ NAA 0. 05 mg/L. The proliferation coefficient was 3.8 and the bud length could reach to 4. 8 cm. When the sturdy test-tube plantlets were inoculated into the medium of 1/2 MS ＋ IBA 0.5 mg/L ＋ NAA 0. 3 mg/L ＋ Citric acid 100 mg/L for rooting induction, the rooting rate became over 95.8%. The transolantin~ survival rate was 87.8% after 35 days.
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