HIF-1α、GLUT-1在子宫内膜癌中的表达及意义

摘要

目的 探讨缺氧诱导因子-1α(HIF-1α)、葡萄糖转运蛋白-1(GLUT-1)在子宫内膜癌中的表达及意义.方法 采用免疫组织化学PV-9000二步法检测36例子宫内膜癌、18例不典型增生、12例子宫内膜复杂性增生、15例子宫内膜单纯性增生组织中HIF-1α、GLUT-1的表达情况.结果 HIF-1α,在子宫内膜单纯性增生、复杂性增生、不典型增生和子宫内膜癌组织中的阳性表达率分别为6.7%、25.0%、38.9%和77.8%.内膜癌组分别与单纯性增生、复杂性增生及不典型增生组比较,差异均有统计学意义(P<0.05).GLUT-1在子宫内膜单纯性增生、复杂性增生、不典型增生和子宫内膜癌组织中的阳性表达率分别为0、8.3%、44.4%和83.3%.子宫内膜癌组分别与单纯性增生、复杂性增生及不典型增生组比较,差异均有统计学意义(P<0.05).子宫内膜病变组织中HIF-1α与GLUT-1表达水平间呈正相关(r=0.559,P<0.01).结论 HIF-1α、GLUT-1在子宫内膜单纯性增生、复杂性增生、不典型增生及子宫内膜腺癌中的阳性表达率逐渐增高,说明HIF-1α及其靶基因GLUT-1可能在子宫内膜病变由良性到恶性转变过程及恶性肿瘤的进展中起重要作用.HIF-1α能提高GLUT-1的表达.%Objective To investigate the expression and significance of hypoxia inducible factor-1 alpha(HIF-1α)and glucose transporter protein 1(GLUT-1)in endometrial carcinoma.Methods Immunohistochemical(PV-9000 two-step)method was applied to detect the expression of HIF-1α and GLUT-1 in 36 cases of endometrial carcinoma,18 cases of atypical hyperplasia,12 cases of complex hyperplasia,15 cases of simple hyperplsia,atypical hyperplasia and endometrial carcinoma were 6.7%,25.0%、38.9%and 77.8%.Compared expression of HIF-1αin endomelrial adenocarcinoma with those in endometrial simple hyperplasia,complex hyperplasia and atypical hyperplasia tissue,differences were statistically significant(P＜0.05).The positive rates of GLUT-1 in endometrial simple hyperplasia,complex hyperplasia,atypical hyperplasia and endometrial adenocarcinoma were 0,8.3%,44.4%and 83.3%.Compared expression of GLUT-1 in endometrial adenocarcinoma with those in endometrial simple hyperplasia,complex hyperplasia and atypical hyperplasia tissue,differences were statistically significant(P＜0.05).According to Spearman Correlation analysis,there was positive correlation between HIF-1α and GLUT-1(r=0.559,P＜0.01).Conclusion The positive rates of HIF-1α and GLUT-1 gradually increase in endometrial simple hyperplasia,complex hyperplasia,atypical hyperplasia and endometrial carcinoma,which indicates HIF-1α and its taget gene GLUT-1 play a very important role in the development of endometrial carcinoma.The expression of GLUT-1 can be enhanced by HIF-1α.