华蟾素与子宫内膜癌HHUA细胞侵袭性生长的实验研究

摘要

目的:探讨华蟾素对体外培养的子宫内膜癌HHUA细胞侵袭性生长的影响和意义.方法:体外培养内膜癌HHUA细胞,以0.25、2.5和25 g/L的华蟾素干预后,四唑盐比色试验检测细胞增殖,流式细胞仪检测细胞凋亡,反转录-聚合酶链反应检测CD44s表达,transwell小室分析细胞的体外侵袭力.结果:华蟾素对内膜癌HHUA细胞的半数抑制浓度(IC50)为华蟾素生药(0.79±0.21) g/L.对照组CD44s mRNA表达为1.31±0.25、侵袭细胞数为60.50±2.52; 0.25 g/L华蟾素对内膜癌HHUA细胞CD44smRNA表达和侵袭力无影响(均P＞ 0.05)；2.5 g/L华蟾素干预后,内膜癌HHUA细胞CD44smRNA表达为1.02±0.23、侵袭细胞数为30.50±3.95,与对照组比较差异有统计学意义(均P＜0.05)；25 g/L华蟾素干预后,内膜癌HHUA细胞CD44s mRNA表达为0.97±0.39、侵袭细胞数为22.50±4.22,与对照组比较差异有统计学意义(均P＜0.05)；25g/L和2.5g/L华蟾素2个浓度组差异无统计学意义(均P＞0.05).结论:2.5 g/L华蟾素可抑制内膜癌HHUA细胞的侵袭性生长.%Objective:To investigate the effect of cinobufagin on the invasive growth of the endometrial carcinoma HHUA cells cultured in vitro. Methods:Endometrial carcinoma HHUA cells were cultured in vitro;after the action of cinobufagin with different concentration, MTT method was used to detect the proliferation of HHUA cells, the cellular apop-tosis was tested by flow cytometry,the expression of CD44s was measured by RT-PCR,the ability of invasion was compared with transwell chamber model. Results:Endometrial carcinoma HHUA cells expressed CD44s mRNA of (1.31±0.25) .number of invasive cell was 60.50±2.52;0.25 g/L cinobufagin didn't affect CD44s and invasive cell(P>0.05); after the action of 2.5 g/L cinobufagin,the expression of CD44s was 1.02±0.23, number of invasive cell was (30.50±3.95),these deta were different from the control group of endometrial carcinoma HHUA cells significantly (P<0.05); after the action of 25 g/L cinobufagin,the expression of CD44s was 0.97±0.39,number of invasive cell was 22.50±4.22,these data were different from the control group of endometrial carcinoma HHUA cells significantly (P<0.05) ;the action of 25 g/L cinobufagin was similar to that of 0.25 g/L cinobufagin(P>0.05). Conclusions:2.5 g/L cinobufagin inhibit the expression of CD44s mRNA and inhibit the invasive growth of the endometrial carcinoma HHUA cells.