高效液相色谱法测定卵巢癌患者血浆中紫杉醇浓度的研究

摘要

Objective:An HPLC method was established for determination of paclitaxel's concentration in ovarian neoplasm patients' plasma.Methods:Each test 500 μL plasma sample was alkalinized by sodium bicarbonate firstly,and then extracted by diethyl ether.Docetaxel was used as the internal standard.Analytical column was Ultimate Acclaim(R) 120 C18 (5 μm,4.6 mm×250 mm).The isocratic mobile phase was the acetonitrile and 0.1％ phosphoric acid with the rate of 55∶45 (V/V).The flow rate was 1.3 mL/min,the temperature of chromatographic column was 20 ℃ and the detective UV wavelength was 227 nm.Results:The calibration curve was linear over the concentration range of 0.1-10.0 mg/L (r=0.9989).The limit of detection was 0.04 mg/L (S/N=3),the relative recovery was (98.5±4.1)％ and the RSD of intra-day and inter-day were lower than 10％ respectively.Conclusions:The method is simple,rapid and repeatable.It is suitable for chemotherapeutic drug monitoring and the pharmacokinetics studying in ovarian neoplasm patients.%目的:建立一种可靠、便捷的高效液相色谱法(HPLC)测定卵巢癌化疗患者血浆中紫杉醇浓度.方法:取500 μL血浆,以多西他赛为内标物,先将血浆样本经碳酸氢钠碱化,再由乙醚萃取.色谱柱为Ultimate Acclaim(R)120 C18(5 μm,4.6 mm×250 mm),流动相为乙腈∶0.1％磷酸水溶液=55∶45(V/V),流速为1.3 mL/min,柱温为20℃,检测波长为227 nm.结果:紫杉醇浓度线性范围为0.1～10.0 mg/L(r=0.998 9),最低检测限为0.04 mg/L[信噪比(S/N)＝3],相对回收率为(98.5±4.1)％,日内和日间相对标准差(RSD)均小于10％.结论:该方法样品处理过程简便、快速,重复性好,可用于卵巢癌化疗患者血浆紫杉醇浓度测定和紫杉醇药物动力学研究.