中药黄芪对人免疫重建荷子宫内膜癌SCID小鼠动物模型的影响

摘要

目的:提取中药黄芪的有效成分黄芪多糖,观察其对人免疫重建荷子宫内膜癌SCID小鼠血清中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-associated lipocalin,NGAL)及其受体NGALR表达的影响.方法:将36只雌性CB-17 SCID小鼠,分为空白对照组、荷瘤组和治疗组,每组各12只.荷瘤组和治疗组先经腹腔注射5×107个/mL人淋巴细胞分离液(PBMC),后肢内侧皮下接种3×106个/mL子宫内膜腺癌细胞株(HEC-1B)建立人免疫重建荷子宫内膜癌模型.成瘤后治疗组按20 mg/kg剂量肌内注射黄芪多糖治疗10 d,空白对照组和荷瘤组注射等量生理盐水.酶联免疫吸附法(ELISA)测定血清NGAL及NGALR蛋白表达,逆转录聚合酶链反应(RT-PCR)检测荷瘤鼠肿瘤组织中NGAL和NGALR基因表达,测量荷瘤组和治疗组小鼠瘤体积、瘤质量并计算肿瘤抑制率.结果:治疗组治疗后与荷瘤组和同组治疗前比较NGAL及NGALR蛋白表达均减弱,NGAL mRNA及NGALR mRNA均低表达,瘤体积和瘤质量均明显降低,抑瘤率提高,差异均有统计学意义(均P<0.05).结论:黄芪多糖可能通过抑制NGAL及NGALR蛋白及其基因的表达而发挥抗肿瘤作用.%Objective:To extract the active ingredients of Astragalus astragalus polysaccharide, observe the immune reconstruction load in endometrial carcinoma SCID mice serum neutrophil gelatinase associated lipocalin (neutrophil gelatinase-associated lipocalin, NGAL) and its receptor NGALR expression influence. Methods:36 female CB-17 SCID mice were divided into blank control group, tumor group and treatment group, tumor group and treatment group were intraperitoneal injection of 5×107/mL Lymphocytes Separation Medium (PBMC), lymphocyte suspension hind leg subcutaneously with 3×106/mL endometrial adenocarcinoma cell line (HEC-1B) establishment of human immune reconstitution in endometrial cancer bearing model. After treatment, the treatment group was injected with astragalus polysaccharide at 20 mg/kg dose for 10 days (d), and the normal control group and the tumor bearing group were injected with normal saline. Enzyme linked immunosorbent assay (ELISA) expression of serum NGAL and NGALR protein were determined by reverse transcriptase polymerase chain reaction (RT-PCR) expression of NGAL gene and NGALR gene detection of tumor tissue, measuring tumor group and treatment group tumor volume, tumor weight and tumor inhibition rate was calculated. Results:After treatment, the treatment group of NGAL and NGALR protein expression decreased, NGAL mRNA and NGALR mRNA were low expression, tumor volume and tumor weight were significantly decreased, the inhibition rate increased, compared with the tumor bearing group and the same group before treatment, the differences were statistically significant (P<0.05). Conclusions:Astragalus polysaccharides may exert antitumor effects by inhibiting the expression of NGAL and NGALR proteins and their genes.