外周神经缺损相关疾病给神经外科医生和患者带来严重困扰。自体神经移植被认为是治疗神经缺损的最佳疗法，但常常受到供体神经束长度等因素的限制。另外，人们在神经组织工程原理的基础上，提出组织-电极接口的设想，用以克服传统神经接口的缺陷。而该假设也是建立在体外获得足够长的神经束的基础上提出的。因此，我们急需建立体外神经组织培育体系，用来获得足够长的神经束。背根神经节是外周神经系统的发源地，是建立体外外周神经培育体系的理想原材料。文中，将提取 SD大鼠背根神经节，并使用左旋多聚赖氨酸或者右旋多聚赖氨酸修饰过的 Aclar膜作为修饰作为背根神经节的生长基底。通过观察背根神经节的生长发育状况，我们发现经右旋多聚赖氨酸修饰的 Aclar膜更适合用于背根神经节的体外培育。%Peripheral nerve injury and amputation have plagued neurosurgeons and patients. Autologous nerve grafts has been known as the “golden rule” to repair severe lesions but is limited by the length and potentially losing functions originally from the donor nerves. Meanwhile, tissue-electrode interface was proposed to overcome the limitation of the traditional neural interface, but long autologous nerves with original functions are preferred. So ifnding out a substitute material to replace the autologous nerves as donor nerves becomes critical. Dorsal root ganglia (DRG) proved an ideal original material. In this report, we described a method to isolate the DRG explants from the spinal cord of SD rat, and coated the Aclar iflms with poly-D-Lysine (PDL) and poly-L-Lysine (PLL) respectively. Then the strategy of culturing the DRG explants on the coated Aclar iflms was introduced. An experiment was designed to investigate the growth of the DRG explants, speciifcally the DRG axons cultured on the two separately coated -Aclar iflms. The results of the experiment show that PDL has an advantage in coating the Aclar iflm for culturing the DRG explants for nerve tissue growth.
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