连花清瘟胶囊中6种有效成分的毛细管胶束电动色谱法测定

摘要

建立了毛细管胶束电动色谱法同时测定中药复方制剂连花清瘟胶囊中甘草苷、芦丁、金丝桃苷、槲皮苷、绿原酸、大黄酸6种药效成分含量的分析方法.使用未涂层弹性石英毛细管,以50 mmol/L SDS-15mmol/L磷酸氢二钠-15 mmol/L硼酸(含25%异丙醇,pH=8.0)作电解质,于254 nm下紫外检测,被测组分在30 min内获得基线分离.甘草苷、芦丁、金丝桃苷、槲皮苷、绿原酸、大黄酸的质量浓度分别在4.5～120、3.0～100、1.5～50、1.5～100、4.5～600、0.90～160 mg/L范围内与峰面积呈良好线性关系,检出限分别为1.5、1.0、0.50、0.50、1.5、0.30 mg/L.样品加标回收率为90%～108%,相对标准偏差均不大于4.2%.方法简便、快速,已成功用于实际样品的分析.%A micellar electrokinetic capillary chromatographic (MEKC) method was established for the simultaneous determination of liquiritin, rutin, hyperin, quercitrin, chlorogenic acid and rhein in Lianhuaqingwen Capsule. Effects of experimental conditions, such as pH value, concentration of sodium dodecyl sulfate( SDS), type of organic modifier, concentration of background electrolyte and applied voltage, on separation efficiency of six targeted compounds were optimized. A baseline separation of six components was achieved in 30 min when the samples were separated on an uncoated fused silica capillary column(75 μm ×60 cm, effective length 50 cm), using 50 mmol/L SDS - 15 mmol/L Na2HPO4 -15 mmol/L H3BO3 (containing 25％ 2-propanol, pH ＝ 8. 0)as running buffer,with applied voltage of 30 kV. The analytes were detected by UV detection at 254 nm. Under the optimum conditions, the calibration curves were linear in the range of 4. 5 - 120 mg/L for liquiritin,3.0 - 100 mg/L for rutin, 1.5 - 50 mg/L for hyperin, 1.5 - 100 mg/L for quercitrin, 4. 5 - 600 mg/L for chlorogenic acid and 0. 90 - 160 mg/L for rhein with detection limits of 1.5, 1. 0, 0. 50,0.50, 1.5, 0. 30 mg/L, respectively. The average recoveries of the six components were between 90％ and 108％ with all relative standard deviations not more than 4. 2％. The developed method was simple, rapid and accurate, and was suitable for the routine analysis of the six effective components in Lianhuaqingwen Capsule.