凝胶净化/超高效液相色谱串联质谱法测定调味酱中32种工业染料

摘要

建立了调味酱中32种工业染料的超高效液相色谱串联质谱分析方法.样品用乙腈均质提取,经凝胶色谱净化,浓缩后,以甲醇定容,超高效液相色谱分离,串联四极杆质谱多反应监测方式监测.结果表明,32种工业染料在各自的线性范围内线性关系良好,相关系数(r)均大于0.994,定量下限为0.2～5.0μg/kg,加标回收率为53％～115％,相对标准偏差为3.9％ ～22.4％.该方法具有前处理简单、净化效果好、灵敏度高的优点,适用于调味酱中多组分工业染料的快速确认和定量检测.%A method for the analysis of 32 industrial dyes in seasoning paste was developed by ultra performance liquid chromatography tandem mass spectrometry(UPLC -MS/MS) combined with gel permeation chromatography(GPC) purification. The homogenized sample was extracted with acetoni-trile, and then purified by GPC. The targeted compounds were detected by MS/MS system with eletrospray ionization(ESI) under multiple reaction monitoring ( MRM ) mode. The result indicated that the limits of quantitation( LOQs) of 32 dyes were in the range of 0. 2 -5 μg/kg. The linear correlation coefficients of 32 dyes were more than 0. 994. The average recoveries ranged from 53% to 115% with RSDs of 3. 9% -22. 4% . This method was suitable for the identification and quantification of multi-component dyes in seasoning paste due to its simplicity, good purification and high sensitivity.